普罗布考的HPLC分析及其在Beagle犬体内的药动学初探

 目的建立血浆中普罗布考浓度的HPLC分析方法,并用此法研究普罗布考在Beagle犬体内的药动学。方法 选择胺碘酮为内标,血浆经异辛烷-异丙醇(8:2)提取,采用Nova-Pak C₁₈(150 mm×3.9 am,4μm)色谱柱,以乙腈-水-10％正丁胺(94:6:0.01)为流动相,流速为1.0 mL·min^-1;在紫外241 nm处检测。所得数据用3P87药动学程序处理,求出有关的药动学参数。结果 普罗布考在0.05～10.0μg·mL^-1内线性关系良好(r=0.999 7),最低检测浓度为0.01μg·mL^-1(S/N≥3)。平均方法回收率为97.8％～103.3％,日内、日间精密度的RSD均小于5％。结论 本方法简便、准确灵敏、重现性好,可用于普罗布考的血药浓度分析及药动学研究。

Study on determination of probucol by HPLC assay and its pharmacokinetics in dogs

OBJECTIVE To establish a HPLC method for the determination of probucol in plasma and to investigate its pharmacokinetic characteristics in dogs.METHODS The drug and internal standard (amiodarone) were extracted from plasma with n-octane-isopropanol(8: 2), and then detected by UV detector at 241 nm with the C₁₈ column and acetonitrile-water-10% n-butylamine(94:6:0.01) as the mobile phase at a flow rate of 1.0 mL·min^-1. The data obtained were processed with 3P87 program on computer. RESULTS The linear range was 0.05～10.0μg·mL^-1 (r=0.9997) .The lowest detectable concentration was 0.01μg·2mL^-1.The mean recoveries of the assay were 97.84%～103.3%. Both the intra-day and inter-day validations were less than 5% .CONCLUSION This method was simple,sensitive,accurate and reproducible. It can be used for the pharmacokinetic studies of probucol in dogs.