菝葜治疗便秘型肠易激综合征作用机制的实验研究

目的研究菝葜治疗便秘型肠易激综合征的疗效及作用机制。方法用冰水灌胃法复制大鼠便秘型肠易激综合征模型,将造模成功大鼠分为模型组、菝葜提取物高、中、低剂量组(浓度依次为8、4、2 g.ml-1,按生药量计)、阳性对照组,另设空白对照组。灌胃给药3周后,酶免法测定大鼠血清5-HT和血浆SS、SP含量的变化,观察菝葜提取物对大鼠结肠5-HT、SP、SS和脊髓SP免疫组化染色的影响,以及对大鼠结肠MC甲苯胺蓝法染色的影响。结果与模型组相比,菝葜提取物低剂量组能够降低模型大鼠血清5-HT和血浆SS含量(P<0.05);能够升高模型大鼠血浆SP的含量(P<0.05);能够升高模型大鼠结肠SS、SP和脊髓SP免疫组化染色的平均灰度值(P<0.05);能够升高模型大鼠结肠5-HT免疫组化染色平均灰度值(P<0.01);并且能够减少模型大鼠结肠MC计数(P<0.05)。结论菝葜对便秘型肠易激综合征模型大鼠有明显的治疗作用,其作用机制可能是通过调整模型大鼠血清5-HT,血浆SS、SP,结肠5-HT、SP、SS、MC,脊髓SP等来实现的。

An experimental study of Smilax treatment of constipation predominant irritable bowel syndrome and its mechanism

Aim To study the effect of Smilax China L.on treating the constipation-predominant irritable bowel syndrome and its mechanism.Method The model was replicated of rats suffering from constipation-predominant irritable bowel syndrome with the method of intragastric administration by ice water and the rats that were made model were successfully divided into the group of model and the extract of Smilax China L.groups of high,middle and low(Concentrations were 8,4,2 g·ml-1 respectively,calculated by the amount of crude drug),as well as active control group and blank control group.The change of content of SS,SP of rat plasma and of 5-HT of rat serum was determined with the enzyme immunoassay and the influence on immunohistochemical staining for 5-HT,SP,SS of rat colon and SP of rat spinal cord as well as the influence on toluidine blue staining for MC of rat colon were observed after three weeksgavage and drug administration.Results The low dose group of extract of Smilax China L.obviously reduced,compared with model group,the content of SS of model rat plasma and of 5-HT of model rat serum(P<0.05),and obviously increased the content of SP of model rat plasma(P<0.05),and obviously raised the value of average gray of immunohistochemical staining for SS,SP of model rat colon and SP of model rat spinal cord(P<0.05),and remarkably raised the value of average gray of immunohistochemical staining for 5-HT of model rat colon(P<0.01),as well as the counting of MC of model rat colon(P<0.05).Conclusion Smilax China L.has evident therapeutic effect on model rat suffering from constipation-dominant irrtable bowel syndrome,which implements its mechanism by adjusting the 5-HT of model rat serum,the SS,SP of model rat plasma,the 5-HT,SS,SP,MC of model rat colon,SP of model rat spinal cord and so on.