化癥散积颗粒对小鼠原位肝癌Bcl-2/Bax表达的影响

目的通过建立小鼠原位肝癌模型,给予化癥散积颗粒灌胃,探讨其对小鼠原位肝癌Bcl-2/Bax表达的影响。方法将H22瘤株直接注射到肝脏的方法建立小鼠原位肝癌模型;分组:模型组、阳性对照组、化癥散积颗粒高、中、低剂量组;采用TUNEL染色法检测凋亡细胞;RT-PCR和Western-Blot分别检测肿瘤组织Bcl-2和Bax mRNA和蛋白表达差异。结果 阳性对照组(又称斑蝥组)和中、高剂量组中药可以诱导肝癌细胞发生凋亡;可以显著增加肿瘤组织中Bax(P<0.05),同时Bcl-2表达与对照组相比明显降低(P<0.05),低剂量组Bcl-2和Bax表达与对照组相比无明显差异(P>0.05)。结论斑蝥组和中、高剂量组中药诱导肝癌细胞发生凋亡可能是通过抑制Bcl-2基因表达,同时增加Bax表达,从而引发一系列凋亡级联反应。

The effects of Huazhengsanjikeli on Bcl-2/Bax expression in mouse of primary Hepatic carcinoma

Objective To study the antitumor mechanism of Granules scattered plot of disease through the insitu-liver cancer model,in order to offer some evidence for clinical Chinese crude drug treatment. Methods Use H22 ceils inject liver to build liver caner moder; Detect apoptosis cells in tumor by TUNEL; Detect the expression difference of Bcl-2/Bax by RT-PCR and Western-blot; Results The number of apoptosis cells were significantly increased in Cantharidin, medium and high dose Granules scattered plot of disease groups than in control and low dose groups; The mRNA and protein expression of Bax can be significantly up-regulation（P〈0.05） in these three groups,but the bcl-2 expression can be obviously inhibited in that three groups（P〈0.05） ; There is no expression difference of Bcl-2 and Bax between control group with low dose Granules scattered plot of disease group（P〉0.05）. Conclusion Cantharidin,medium and high dose Granules scattered plot of disease can induce H22 liver caner cells to apoptosis; Granules？ scattered plot of disease can be induce liver cancer cells to apoptosis through inhibited the expression of Bcl-2 and up-regulated the expression of Bax at the same time.