| 松果体素抑制大鼠海马神经元甘氨酸激活的全细胞电流 |
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| 引用本文： | 程新萍,开远忠,吴旭,刘雅静,周江宁. 松果体素抑制大鼠海马神经元甘氨酸激活的全细胞电流[J]. 中国药理学通报, 2012, 28(1): 97-100. DOI: 10.3969/j.issn.1001-1978.2012.023. |
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| 作者姓名： | 程新萍 开远忠 吴旭 刘雅静 周江宁 |
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| 作者单位： | 1. 中国科学技术大学生命科学学院,安徽,合肥,230027
2. 中国科学技术大学生命科学学院,安徽,合肥,230027;安徽大学生命科学学院,安徽,合肥,230039 |
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| 基金项目： | 安徽省自然科学基金资助项目(No 090413270);中国科学技术大学校青年创新基金资助项目(No WK2070000009) |
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| 摘 要： | 目的研究松果体素对培养的大鼠海马神经元甘氨酸激活的全细胞电流(Igly)的调控。方法在培养的大鼠海马神经元上,采用全细胞膜片钳技术,研究松果体素对甘氨酸激活的全细胞电流的调控。结果松果体素以浓度依赖的方式可逆地抑制Igly,并且这种抑制作用是非竞争性的,松果体素的抑制作用没有特异的亚基选择性。结论松果体素能以非竞争的方式直接抑制甘氨酸受体介导的电流,这种抑制作用可能对海马区域神经网络的兴奋性产生影响。
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| 关 键 词： | 松果体素 甘氨酸受体 全细胞膜片钳技术 海马神经元 大鼠 甘氨酸 海马 |
Inhibition of glycine-activated current by melatonin in cultured rat hippocampal neurons |
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| CHENG Xin-ping,KAI Yuan-zhong,WU Xu,LIU Ya-jing,ZHOU Jiang-ning. Inhibition of glycine-activated current by melatonin in cultured rat hippocampal neurons[J]. Chinese Pharmacological Bulletin, 2012, 28(1): 97-100. DOI: 10.3969/j.issn.1001-1978.2012.023. |
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| Authors: | CHENG Xin-ping KAI Yuan-zhong WU Xu LIU Ya-jing ZHOU Jiang-ning |
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| Affiliation: | 1(1.School of Life Sciences,University of Science and Technology of China,Hefei 230027,China; 2.School of Life Sciences,Anhui University,Hefei 230039,China) |
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| Abstract: | Aim To investigate the effect of melatonin on the glycine-activated current(Igly) in cultured rat hippocampal neurons.Method Whole-cell patch-clamp technique was used.Result Melatonin rapidly and reversibly inhibited the amplitude of glycine-activated current mediated by native glycine receptors and recombinant α1-、α2-、α3-and α1β-glycine receptors.In cultured rat hippocampal neurons,melatonin inhibited the glycine-activated current in a dose-dependent manner.Furthermore,melatonin noncompetitively inhibited the Igly.Conclusion Melatonin can directly depress GlyR-mediated currents in a non-competitive and subunit-independent manner,suggesting that melatonin may modulate glycinergic transmission and hippocampal network activity. |
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| Keywords: | melatonin glycine receptor whole-cell patch-clamp technique hippocampal neuron rat glycine hippocampus |
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