维生素E对鼠海马神经元细胞的抗氧化损伤作用

目的观察维生素E对老年痴呆(Alzheimer'sdisease)的治疗作用,研究氧化损伤对鼠海马神经元细胞的损伤作用及维生素E对其氧化损伤的保护作用。方法用氧化(H2O2)、维生素E处理后氧化的方法对鼠海马神经细胞(HT-22)进行分组处理。用二维电泳法、蛋白银染法及特殊氧化蛋白免疫染色法探测被氧化的蛋白。结果氧化处理12h后出现细胞生存率明显下降31.19%;经维生素E处理后再氧化处理的细胞生存率几乎达到正常组水平。经H2O2氧化处理的鼠海马神经元细胞的氧化蛋白数目增加了,而维生素E提前处理过的那组没有增加。结论维生素E对鼠神经元细胞的氧化损伤起保护作用,是有效的抗氧化治疗剂。

Vitamin E prevents oxidation of protein in rats hippocampal neuronal cells

AIM:To observe effect of Vitamin E on Alzh eimer' s disease and st ud y oxidative damage to mouse hippocamp al neuronal cells proteins and prevent ef fect of Vitamin E on oxidative dama ge.METHODS:Using oxidative stress(H 2 O 2 )and vitamin E pretreated plus oxidative stress(H 2 O 2 )to mouse hippocampal neuronal cell l ine(HT-22).Ox-idized proteins by 2-dimensional ge l electrophoresis with silver stain ing and oxidation-spe cific immunostains were measured.RESULTS:After oxidative stress 12hours,sur vival rate markedly decreases to 31.19%.Cells survival rate nearly reach ed normal level after Vitamin E treatment followed by oxidation tre atment.The number of oxidative proteins in H 2 O 2 -treated hippocampal neurons incre ased,while no increase was found in Vit amin E treated neurons.