几丁糖/海藻酸敷料止血性能的实验研究

目的研究一种新型敷料：几丁糖／海藻酸敷料（本项目组自行研制，已申请国家专利）的止血性能。方法取新西兰兔4只，在背部两侧对称性剪5个直径1cm的圆型创口，分别与创面大小相当的几丁糖／海藻酸敷料和明胶海绵止血，观察与创面的粘附情况，记录出血时间：止血停止后，将几丁糖／海藻酸敷料和明胶海绵放入预先配制好的氰化高铁血红蛋白检测试剂中仔细清洗，用分光光度计在540nm波长处光度比色，测出的Hb光度吸收值表示出血量。结果几丁糖／海藻酸敷料与创面粘附较好，几丁糖／海藻酸敷料、明胶海绵组的出血时间分别为87.7±19.1妙、170.7±22.6妙，Hb光度吸收值分别为1.131±0.44、1.733±0.733，经统计学分析，两组数据都有显著性差异（P〈0.01），几丁糖／海藻酸敷料组明显优于明胶海绵组。结论几丁糖／海藻酸敷料具有较好的止血性能。

Experimental study of Chitosan/alginate dressings'hemostatic properties

Objective To study the hemostatic properties of a novel dressing, which is made from chitosan and alginate （developed by our project team, have applicated national patent）. Methods Bleeding time were measured for bilateral back incisions（5 incisions on each side）in 4 New Zealand white rabbits. Using a randomized,blinded experimental design, one incision in each animal was treated with chitosan/alginate dressings, the other was treated with gelatin sponge. After bleeding stopped, put the chitosan/alginate dressings and gelatin sponge into cyanmethemoglobin agent and carefully cleaned, absorbance was detected by spectrophotometer at 540 nm wavelength, which behalf of the amount of bleeding.Results The group of chitosan/alginate dressings adhered to incision better than the gelatin sponge group, the bleeding time ofchitosan/alginate dressings group and gelatin sponge group were 87.7±19.1 seconds and 170.7±22.6 seconds, respectively. Hb absorbance value were 1.131±0.44 and 1.733±0.733, respectively, the two groups of data have significant difference by statistical analysis （P〈0.01）, Chitosan/alginate dressings group is significantly better than gelatin sponge group. Conclusion Chitosan/alginate dressings hemostasis performance is better.