化疗前后B-NHL淋巴瘤IgH基因重排变化的研究

目的B—NHL是我国较多见的肿瘤之一。通过对B—NHL患者化疗前后IgH基因重排变化的观察，探讨IgH基因单克隆重排带对B—NHL的诊断价值及能否作为B—NHL化疗缓解后的分子指标之一。方法对20例B—NHL初治化疗前及化疗缓解后患者用两种PCR方法进行IgH基因重排物的检测，同时用10例非淋巴瘤肿瘤组化疗前及化疗缓解后患者及10例健康人以作对照。结果20例初治化疗前B—NHL患者18例检测到IgH基因单克隆重排带，化疗缓解后此18例中1例IgH基因单克隆重排带转阴，另出现2或3克隆性重排带各2例，总变化率为27．8％，余未变化。2例IgH基因单克隆重排带阴性者化疗后依然阴性。对照的非淋巴瘤肿瘤组化疗前及化疗缓解后患者及健康人IgH基因单克隆重排带均为阴性，患者治疗后也未见改变。结论IgH基因单克隆重排带，可以作为B—NHL及其微小残留灶克隆性变化的辅助诊断指标之一。

Study of IgH gene rearrangement in B-NHL before and after chemical therapy

Objective： To study the change of IgH gene rearengement in B - NHL before and after chemical therapy. Methods： Use 2 types of PCR methods to check B - NHL 20 cases, Non - NHL tumor 20 cases, normal people 10 cases. Results ： Before chemi- cal therapy in 20 B - NHL cases, 18 cases had IgH gene rearangemen one - band, after chemical therapy 1 cases had negotive IgH gene rearrangement one - band, 2 cases had 2 bands, 2 cases had 3 bands. Other 2 groups had no IgH gene rearrangement band. Conclusion ： IgH gene rearangement one - band could be a method for diagnosis of B - NHL and its MRD changes.