Pollen mitosis and pollen tube growth inhibition by SO2 in cultured pollen tubes of Tradescantia |
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Authors: | Te-Hsiu Ma Shaukat H Khan |
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Institution: | Department of Biological Sciences, Western Illinois University, Macomb, Illinois 61455 USA |
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Abstract: | Mature pollen of Tradescantia paludosa Anders. Sax clone-3 was collected from greenhouse grown plants and desiccated in the dark for 4–5 hours. The pollen was sown on microslides coated with lactose agar medium (supplemented with 0.02% colchicine). Five minutes after sowing, pollen cultures were treated with various concentrations (0.075–2000 ppm) of SO2 obtained from the chemical reaction between K2S2O5 and HCl. Treatments were carried out in an airtight Plexiglas chamber throughout the full incubation period of 19 hours. Treated and control pollen cultures were fixed and stained in aceto-carmine or Feulgen reaction to determine the tube lengths and mitotic indices. Results of repeated experiments indicated that the values of mitotic indices dropped from the normal 38.7% in the control to the range of 24.3-3.8% when treated with 0.075–50.00 ppm of SO2. Pollen tube growth was inhibited from the normal length of 1.5 mm in the control to the range of 800-100 μm when treated with 10–2000 ppm of SO2. The inhibitory effect of SO2 rpresumably resulted from physiological disturbance of the cell and severe damage to chromosomes of both generative and tube nuclei of the pollen tubes. |
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