生长因子对人视网膜神经胶质细胞增殖的作用

目的：研究生长因子对培养的人视网膜神经胶质细胞增殖的作用。方法：在培养的人视网膜神经胶质细胞中分别加入表皮生长因子(epidermal growth factor, EGF,0.5～100.0ng/ml)和神经生长因子(nerve growth factor,NGF,0.05～10.0ng/ml),培养3天后用MTT法测量细胞增殖情况。结果：EGF(0.5～100.0ng/ml)和NGF(0.05～10.0ng/ml)均能刺激人视网膜神经胶质细胞的增殖,其EC₅₀(half effective concentration,半数有效浓度)分别为17.0ng/ml和 0.7ng/ml。结论：EGF和NGF均能刺激人视网膜神经胶质细胞的增殖,它们可能在增殖性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR）等增殖性病变中起一定作用。 (中华眼底病杂志,1998,14:33-34) (中华眼底病杂志,1998,14:33-34)

Growth factor effects on the proliferation of human retinal glial cell in culture

Objective:To study the effects of growth factor on the proliferation of the cultured huamn retinal glial cells. Methods:EGF(0.5～100.0ng/ml) and NGF (0.5～10.0ng/ml) were added to cultures of human retinal glial cells and the proliferation rates of the cells were measured by MTT method. Results:EGF at a dosage ranging from 0.5ng/ml to 100.0ng/ml and NGF (0.05～10.0ng/ml) stimulated the cellular proliferation effectively with their EC 50 of 17ng/ml and 0.7 ng/ml respectively. Conclusion:Both EGF and NGF NGF had an effective stimulation on human retinal glial cell proliferation.They may play a role in the formation of PVR.(Chin J Ocul Fundus Dis,1998,14:33-34)