附子与甘草不同配伍比例配伍减毒的实验研究

目的:通过平行比较附子及附子与甘草不同配伍比例对附子毒性的影响,观察甘草对附子的解毒作用,并对减毒机制进行探索性的研究。方法:平行比较了附子和附子配伍不同比例甘草对附子小鼠急性毒性,测定其半数致死剂量LD50;平行比较附子和附子配伍甘草对大鼠的心脏毒性,测定其半数中毒剂量TD50;以无血清DMEM将附子、附子-甘草3∶1、附子-甘草1∶1、附子-甘草1∶3的含药血清分别按5%,10%,20%稀释成3个浓度,观察各给药组不同浓度的含药血清对原代乳大鼠心肌细胞搏动节律、细胞存活率以及细胞内乳酸脱氢酶(LDH)含量的影响。结果:附子配伍甘草后能够提高附子的LD50和TD50。同空白血清对照组比较附子含药血清能够明显增加心肌细胞搏动节律和LDH的含量(P<0.05),附子配伍不同比例甘草含药血清能够明显降低附子导致心机搏动节律的加快,降低LDH含量。随着甘草比例的增加,作用加强。但不同浓度的附子以及附子配伍甘草含药血清对细胞存活没有明显影响。结论:甘草能够通过提高附子中毒剂量,从而达到减毒作用。甘草对于附子减毒作用是通过抑制心肌细胞节律的增加,保护心肌细胞从而达到减毒作用。

Detoxication experimental study on different compatibility proportion of Aconiti Lateralis Radix Praeparata and Glycyrrhizae Radix et Rhizoma

Objective: Through the paired comparison on the toxicity effect of Aconiti Lateralis Radix Praeparata of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Glycyrrhizae Radix et Rhizoma,to observe the detoxication effect of Glycyrrhizae Radix et Rhizoma to Aconiti Lateralis Radix Praeparata.Method: Paired comparison on the mouse acute toxicity of Aconiti Lateralis Radix Praeparata and Aconiti Lateralis Radix Praeparata with different compatibility proportion of Glycyrrhizae Radix et Rhizoma,to assay the LD50.Paired comparison on the rat heart toxicity of Aconiti Lateralis Radix Praeparata and Aconiti Lateralis Radix Praeparata with different compatibility proportion of Glycyrrhizae Radix et Rhizoma,to assay the TD50.We dilute medicated serum of Aconiti Lateralis Radix Praeparata,Aconiti Lateralis Radix Praeparata plus Glycyrrhizae Radix et Rhizoma(3∶1),Aconiti Lateralis Radix Praeparata plus Glycyrrhizae Radix et Rhizoma(1∶1) into 5%,10%,20% solution with serum free DMEM,to survey the effect of different concentration of medicated serum to the pulsing rhythm of myocardial cell of original generation newborn rat,cell surviva rate and content of LDH in myocardial cells.Result: LD50 and TD50 of Aconiti Lateralis Radix Praeparata can be increased after adding Glycyrrhizae Radix et Rhizoma.Compared to the blank serum,medicated serum with Aconiti Lateralis Radix Praeparata can obviously increased the pulse rhythm of myocardial cell and the content of LDH(P< 0.05).The medicated serum with Aconiti Lateralis Radix Praeparata added different proportion of Glycyrrhizae Radix et Rhizoma can reduce the acceleration of myocardial cell′s rhythm,which is induced by Aconiti Lateralis Radix Praeparata,and can reduce the content of LDH.With the increased ratio of Glycyrrhizae Radix et Rhizoma,the effect is stronger.But for the serum with different concerntration of Aconiti Lateralis Radix Praeparata or Aconiti Lateralis Radix Praeparata added Glycyrrhizae Radix et Rhizoma,there is no obvious effect to the cell survival.Conclusion: Glycyrrhizae Radix et Rhizoma has the detoxication effect through increasing the ultimatetotaldosage of Aconiti Lateralis Radix Praeparata.The detoxication effect of Glycyrrhizae Radix et Rhizoma to Aconiti Lateralis Radix Praeparata is through restraining the increased rhythm of myocardial cell and protecting the myocardial cell.