壁虎体外抗Bel-7402肿瘤细胞活性部位的筛选研究

目的:对壁虎体外抗Bel-7402肿瘤细胞的活性部位进行筛选研究,为壁虎抗肿瘤活性物质研究奠定基础。方法:采用超声﹑萃取的方法依次分级提取壁虎的石油醚部位、二氯甲烷部位、乙酸乙酯部位、正丁醇部位和水部位,并采用噻唑蓝(MTT)法对不同极性部位的体外抗Bel-7402肿瘤细胞作用进行研究。结果:壁虎醇提物的石油醚部位、二氯甲烷部位、乙酸乙酯部位、正丁醇部位和水部位在体外对Bel-7402肿瘤细胞抑制率分别为93.24%、74.81%、93.25%、91.03%和25.13%,抑制率从高到低依次为石油醚部位、乙酸乙酯部位、正丁醇部位、二氯甲烷部位、水部位。结论:壁虎醇提物的石油醚部位、二氯甲烷部位、乙酸乙酯部位、正丁醇部位和水部位对Bel-7402肝癌细胞均有一定抑制作用,其中以石油醚部位的抑制作用最强。

Screening Active Fractions of Gekko against BEL-7402 Cells in Vitro

Objective:To screen the active fraction against BEL-7402 cells in vitro,and to lay a foundation for the study of anti-tumor active substances in gekko. Method:The petroleum ether fraction,methylene chloride fraction,ethyl acetate fraction,n-butanol fraction and water fraction of gekko were extracted by ultrasonic and extraction methods,and the in vitro anti-BEL-7402 cells of different polar sites was determined by MTT method. Results:The inhibition rates for Bel-7402 cells in the petroleum ether fraction,methylene chloride fraction,ethyl acetate fraction,n-butanol fraction and water fraction of gekko alcohol extract were93.24%,74.81%,93.25%,91.03% and 25.13%,respectively. Among them,on the inhibition rates,the petroleum ether fraction(100 μg/mL) was the strongest,followed by the ethyl acetate fraction,the n-butanol fraction,the dichloromethane fraction and the water fraction. Conclusion:In vitro,petroleum ether,methylene chloride,ethyl acetate,n-butanol and water have a certain inhibitory effect on BEL-7402 cells,in which the petroleum ether fraction has the strongest effect.