三仁汤对湿热证型病毒性肺炎模型小鼠免疫功能的干预作用

目的:探讨三仁汤对湿热证型病毒性肺炎模型小鼠免疫功能的干预作用。方法:将55只小鼠随机分为空白组、模型组、三仁汤组、利巴韦林组,其中空白组10只,其余3组每组15只。除空白组外其他3组通过肥甘饮食、湿热环境、流感病毒感染三因素合并,构建湿热证型病毒性肺炎动物模型。滴鼻感染H1N1病毒2 h后灌胃给药,每天2次,连续给药6 d后处死动物,观察并记录小鼠肛温、体质量;苏木精-伊红染色法(HE)观察小鼠肺组织病理变化;酶联免疫吸附法检测血清中白细胞介素-1(IL-1)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)浓度;流式细胞仪法检测全血中CD3+、CD3+/CD4+、CD3+/CD8+含量。结果:与模型组相比,三仁汤组小鼠血清中IFN-γ、IL-1水平升高,IL-4水平降低(P0.05);外周血中CD3+、CD3+/CD4+恢复良好,达空白组水平(P 0.05),CD3+/CD8+含量有升高(P0.05)。结论:三仁汤具有改变机体炎性因子含量、增强机体免疫功能的作用。

Intervention Effects of Sanren Decoction on Immune Function of Model Mice with Virus Pneumonia of Dampness-heat Syndrome

Objective:To investigate the intervention effects of Sanren Decoction on the immune function of model mice with virus pneumonia of dampness-heat syndrome. Methods:Total 55 mice were randomly divided into the blank group,model group,Sanren Decoction group and ribavirin group,10 mice in the blank group and 15 mice in other 3 groups. In addition to the blank group,the other three groups were combined with three factors of fat diet,hot and humid environment and influenza virus infection to construct the model mice with virus pneumonia of dampness-heat syndrome. After intranasal infection with H1 N1 virus for 2 hours,the rats were intragastrically administered twice a day. After 6 days of continuous administration,the animals were sacrificed. The anus temperature and body weight of the mice were observed and recorded. Histopathological changes of the lungs were observed by hematoxylin-eosin staining(HE). Serum interleukin-1(IL-1),interleukin-4(IL-4),interferon-γ(IFN-γ) concentrations were detected by enzyme-linked immunosorbent assay. The content of CD3+,CD3+/CD4+and CD3+/CD8+in whole blood was detected by flow cytometry. Results:Compared with the model group,Sanren Decoction group could increase the levels of IL-1,FN-γ and decrease the levels of IL-4 in mice serum(P< 0.05). CD3+and CD3+/CD4+recovered well,reaching the level of blank group(P> 0.05). The contents of CD3+/CD8+increased. Conclusion:Sanren Decoction can change the body's inflammatory factor content and enhance the body's immune function.