儿童分泌性中耳炎耳积液中免疫相关指标表达分析

目的 探讨儿童分泌性中耳炎患者中耳积液中的免疫相关指标变化情况。 方法 选取2016年12月至2017年12月收治的30例分泌性中耳炎患儿设为研究组,选取同期体检的健康儿童30例设为对照组。比较两组外周血中CD4⁺T、CD8⁺T细胞百分数及CD4⁺ /CD8⁺比值。比较研究组中耳积液及血浆中IL-2、IL-4、IL-6水平。将研究组外周血CD4⁺,CD8⁺T细胞值与中耳积液中IL-2、IL-4、IL-6值进行相关性分析。 结果 研究组外周血中CD4⁺T、CD8⁺T细胞百分数均明显高于对照组,CD4⁺ /CD8⁺比值明显低于对照组,差异有统计学意义(P<0.01)。研究组中耳积液中IL-2、IL-4、IL-6水平均明显高于血浆,差异有统计学意义(P<0.01)。研究组血浆中IL-2、IL-4、IL-6水平均明显高于对照组,差异有统计学意义(P<0.01)。Pearson直线相关分析结果显示,研究组中耳积液中IL-2、IL-4、IL-6水平与外周血CD4⁺T、CD8⁺T含量均呈显著正相关(P<0.01)。 结论 分泌性中耳炎与强烈的免疫反应密切相关,存在CD4⁺T、CD8⁺T细胞亚群显著升高的现象,IL-2、IL-4、IL-6对于儿童分泌性中耳炎的诊断具有一定的临床意义。

Expression of immunological markers in middle ear effusion in children with secretory otitis media

Objective To evaluate the expression of immunological markers in middle ear effusion in children with secretory otitis media. Methods The research group included 30 patients with secretory otitis media, who were treated in our hospital from December 2016 to December 2017, while 30 healthy children were selected as the control group. The percentages of CD4⁺T and CD8⁺T cells and the CD4⁺/CD8⁺ ratio in the peripheral blood of the two groups were compared. The levels of IL-2, IL-4, and IL-6 in the middle ear effusion and plasma between the two groups were compared. The correlation of CD4⁺T and CD8⁺T cell values and IL-2, IL-4, and IL-6 in middle ear effusion was analyzed. Results The percentages of CD4⁺T and CD8⁺T cells in peripheral blood of the study group were significantly higher than that in the control group, and the CD4⁺/CD8⁺ ratio was significantly lower than that in the control group with a statistically significant difference(P<0.01). The levels of IL-2, IL-4, and IL-6 in the middle ear effusion of the group were significantly higher than that in the plasma, and the difference was statistically significant(P<0.01). The levels of IL-2, IL-4, and IL-6 in the study group were significantly higher than those in the control group, and the difference was statistically significant(P<0.01). Pearson linear correlation analysis showed that the levels of IL-2, IL-4, and IL-6 in the middle ear effusion of the group had a significantly positive correlation with CD4⁺T and CD8⁺T cell values in peripheral blood(P<0.01). Conclusion Secretory otitis media is closely related to the strong immune response and the phenomenon of significant rise in the CD4⁺T and CD8⁺T cell subgroup. Additionally, IL-2, IL-4, IL-6 showed a certain clinical significance in diagnosing and managing secretory otitis media in children.