可溶性细胞间黏附分子-1和可溶性选择素E在自身免疫性风湿病中的表达

目的了解自身免疫性风湿病患者血清中两种可溶性黏附分子(sAM)——可溶性细胞间黏附分子-1(sICAM-1)和可溶性选择素E(sE-selectin)的水平,并揭示两种sAM与临床病情的关系。方法采用酶联免疫吸附试验(ELISA)检测三种自身免疫性风湿病系统性红斑狼疮(SLE)、类风湿关节炎(RA)、多发性肌炎/皮肌炎(PM/DM)患者,30例非自身免疫性风湿病患者及20名健康对照血清中sICAM-1、sE-selectin的含量,并分析两种sAM与临床资料、实验室检测指标的相关性。结果所测自身免疫性风湿病患者(包括3个组)血清中sICAM-1、sE-selectin水平较非自身免疫性风湿病及正常对照组明显增高(P<0.05)。RA组患者血清中sICAM-1与类风湿因子(RF)呈正相关。PM/DM组患者血清sE-selectin水平与肌酸激酶(CK)值呈正相关。结论本研究结果表明,sICAM-1和sE-selectin参与了自身免疫性风湿病的发病过程。异常升高的sICAM-1、sE-selectin水平可反映某些类型的自身免疫性风湿病(RA、PM/DM)的活动程度。

Concentrations of sICAM -1 and sE-selectin in sera from patients with autoimmune rheumatic disease

Objective To evaluate the sera level of soluble intercellular adhesion molecule-1 (sICAM-1) and soluble E-selectin (sE-selectin) in patients with rheumatic diseases and to explore the correlation between the sera level of soluble adhesion molecules (sAM) and clinical findings. Methods The concentrations of sICAM-1 and sE-selectin in the sera of patients with rheumatic diseases (SLE, RA, PM/DM) and patients without rheumatic diseases, as well as the healthy controls were measured by the enzyme-linked immunosorbent assay (ELISA). After that, the correlation of the concentrations of sAM and clinical data as well as other laboratory parameters (RF, CK, anti-dsDNA) were analyzed. Results The concentrations of sICAM-1 and sE-selectin in all three group patients were significantly higher than those of non-autoimmune rheumatic diseases and healthy controls (P<0.05). The correlation could be found between the concentration of sICAM-1 and RF in RA groups. Good correlation was also found between the level of sE-selectin and CK in PM/DM group. Conclusion This investigation strongly suggests that sICAM-1 and sE-selectin is involved in the inflammatory process of rheumatic diseases, and the abnormally high concentrations of sICAM-1 and sE-selectin may reflect the activity of some rheumatic diseases (RA, PM/DM).