CHARACTERIZATION OF RAT TESTICULAR ALCOHOL DEHYDROGENASE |
| |
Authors: | CHIAO, YU-BIN VAN THIEL, DAVID H. |
| |
Affiliation: | Department of Medicine, University of Pittsburgh School of Medicine Pittsburgh, PA 15261, U.S.A. |
| |
Abstract: | A protein from rat testes that catalyzes the oxidation of ethanolin the presence of NAD+, but not NADP+, has been characterizedenzymatically and compared to that of hepatic alcohol dehydrogenaseobtained from the same animals. The testicular enzyme, likethe hepatic enzyme, has a Km value for ethanol in the 0.51.0-mMrange and can utilize other alcohols such as n-propanol, n-butanol,and isobutanol, although the Km values for these other alcoholsare considerably lower (0.030.08 mM) that that for ethanol.The testicular enzyme is more heat-labile than is the hepaticenzyme. Finally, the testicular enzyme catalyzes the oxidationof retinol and its retinol dehydrogenase activity is inhibitedby ethanol. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|