| 丹参素的抗癌活性研究 |
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| 引用本文： | 赵广荣,田莉莉,王长松.丹参素的抗癌活性研究[J].药物评价研究,2010(3):180-182. |
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| 作者姓名： | 赵广荣 田莉莉 王长松 |
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| 作者单位： | 系统生物工程教育部重点实验室,天津市生物与制药工程重点实验室,天津大学化工学院制药工程系,天津300072 |
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| 基金项目： | 国家自然科学基金（30873400）; 国家重大新药创制专项资助（2009ZX09301） |
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| 摘 要： | 目的：建立体外抗癌天然药物筛选模型,评价丹参素的抗癌活性。方法：以遗传物质DNA为靶标,溴化乙锭（EB）为致癌物质,丹参素为试药,利用荧光光谱检测丹参素对EB-DNA体系的荧光猝灭效应;利用圆二色谱技术检测丹参素对EB-DNA体系的干扰作用。结果：丹参素能明显抑制EB-DNA体系的荧光强度,且具有浓度依赖性;丹参素能够有效阻断EB嵌入DNA的碱基对中,维持DNA分子的正常构象。结论：丹参素抑制致癌剂对DNA链的嵌合,从而起抗癌活性。该模型可应用于抗癌功效的天然药物的筛选中。
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| 关 键 词： | 筛选模型 抗癌天然药物 丹参素 荧光光谱 圆二色谱 DNA构象 |
Anticancer activity of denshensu |
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| ZHAO Guang-rong,TIAN Li-li,WANG Chang-song.Anticancer activity of denshensu[J].Drug Evaluation Research,2010(3):180-182. |
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| Authors: | ZHAO Guang-rong TIAN Li-li WANG Chang-song |
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| Institution: | ( Key Laboratory of Systems Bioengineering,Ministry of Education;Tianjin Key Laboratory of Biological and Pharmaceutical Engineering;Department of Pharmaceutical Engineering,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,P.R.China ) |
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| Abstract: | Objective：To establish in vitro screening model for natural anticancer drugs and evaluate the anticancer activity of denshensu on carcinogenic damaged DNA.Methods：Genetic material DNA was used as target,ethidium bromide（EB） as carcinogen,quenching effect of denshensu on fluorescence spectrum of EB-DNA was detected.Interfering effect of denshensu on circular dichroism spectra of EB-DNA was measured.Results：Denshensu strongly quenched the fluorescent intensity at a dose-dependent manner.The circular dichroism spectra showed that denshensu could inhibit the insertion of EB to base pairs of DNA,and maintain the normal conformation of DNA.Conclusion：Denshensu possesses strong anticancer activity by inhibiting insertion of carcinogens into genetic DNA.This model would be applicable for screening natural anticancer drugs |
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| Keywords: | screening model anticancer natural drug denshensu fluorescence spectrum circular dichroism spectr um DNA conformation |
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