| 红缘拟层孔菌液体发酵培养基配方的研究 |
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| 引用本文： | 马琳,徐田田,张铁军.红缘拟层孔菌液体发酵培养基配方的研究[J].药物评价研究,2010(2):121-124. |
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| 作者姓名： | 马琳 徐田田 张铁军 |
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| 作者单位： | [1]天津中医药大学,天津300193 [2]天津药物研究院,天津300193 |
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| 摘 要： | 目的：研究不同培养基组成成分对红缘拟层孔菌菌丝产量和多糖量的影响，优选出的红缘拟层孔菌液体发酵培养基的最佳配方。方法：以菌丝体生物量和胞内多糖的量为指标，通过单因素试验确定液体培养基中最佳碳源、氮源及维生素B1的用量。通过L9（3^4）正交试验确定红缘拟层孔菌的液体发酵培养基配方的最佳组合。结果：优选出的红缘拟层孔菌液体发酵培养基最佳配方为（g／100mL）：大米粉5，酵母粉1，磷酸二氢钾0．1，硫酸镁0．15，维生素B10．04。结论：优选出红缘拟层孔菌的液体发酵培养基的最佳配方，为今后大规模液体发酵研究奠定了基础。
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| 关 键 词： | 红缘拟层孔菌 液体发酵 正交设计试验 多糖 |
Study on culture medium composition for liquid fermentation of Fomitopsis pinicola |
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| MA Lin,XU Tian-tian,ZHANG Tie-jun.Study on culture medium composition for liquid fermentation of Fomitopsis pinicola[J].Drug Evaluation Research,2010(2):121-124. |
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| Authors: | MA Lin XU Tian-tian ZHANG Tie-jun |
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| Institution: | 1 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; 2 Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China) |
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| Abstract: | Objective： To study the optimal culture medium composition for the liquid fermentation of Fomitopsispinicola. Methods： Taking the mycelial biomass and production of polysaccharide as detecting indices, the optimum submerged fermentation parameters for F. pinicola, were studied by single factor test and orthogonal test. Results： The optimal formula for liquid fermentation （g/100 mL） were： rice powder 5, yeast 1, KH2PO3 0.1, MgSO4 0.15, and VB1 0.04. Conclusion： The optimal medium composition for the liquid fermentation ofF. pinicola has been selected, which provides the scientific basis for the further research of F. pinicola. |
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| Keywords: | Fomitopsis pinicola liquid fermentation orthogonal test polysaccharide |
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