微波法提取当归中阿魏酸及HPLC测定其含量的研究

目的：建立当归中阿魏酸的提取及含量测定方法，为当归的质量标准控制提供理论依据。方法：采用微波提取当归中阿魏酸，萃取液为80％甲醇20ml，微波萃取仪功率：为最大功率（850W）的25％；时间：恒压时间15min，总时间25min；压力值0．40MPa。采用高效液相色谱（HPLC）法测定当归中阿魏酸的含量，Hitachi7000型高效液相色谱仪，Symmetery-C18（4．6mm×150mm，5μm）色谱柱；柱温：35℃；流动相：乙晴-0．02mol／l磷酸二氢钾溶液（17：83，pH2．5）；流速：1．0ml／min；检测波长：322nm；进样体积20μl。结果：阿魏酸进样量在0．048-0．128μg范围内与峰面积呈良好线性关系，R=0．9998；平均回收率为100．01％，RSD=2．41％。结论：本实验所建立的方法专属性强，准确度高，重现性好，可作为当归的质量控制方法。

In the microwave method extraction Chinese angelica the ferulic acid and HPLC determine its content the research

Objective： Establishes in the Chinese angelica the ferulic acid extraction and the content determination method, provides the theory basis for the Chinese angelica quality specification control. Methods：Uses in the microwave extraction Chinese angelica the ferulic acid, the extracted liquid is 80% methyl alcohol 20ml, the microwave extract meter power： For maximum work rate （850W） 25% ; Time： Constant pressure time 15min, total time 25min; Value of pressure 0.40MPa. Uses the highly effective liquid chromatography （HPLC） in the law determination Chinese angelica the ferulie acid content, the Hitachi 7000 highly effective liquid phase chromatographs, Symmetery- C18 （4. 6mm × 150mm, 5μm） the chromatographic column; The column is warm： 3522 ; Flowing： Second grade clear - 0.02mol/1 potassium dihydrogen phosphate solution （17：83, pH2. 5）; Speed of flow： 1. 0ml/min; Examination wave length： 322nm; Specimen handling volume 20td. Results： The ferulic acid specimen handling quantity assumes the good linear relationship in 0.048-0. 128 μg scope with the peak area, R=0. 9998; The average returns-ratio is 100. 01 %, RSD= 2.41%. Conclusion：This experiment establishes method specificity, the accuracy is high, the reproduction quality is good, may take the Chinese angelica the quality control method.