高葡萄糖在正常氧及缺氧条件下对乳鼠心肌细胞存活与凋亡的不同影响

目的探讨高葡萄糖在正常氧及缺氧条件下对乳鼠心肌细胞存活与凋亡的影响。方法原代培养乳鼠心肌细胞,予33mmol/L葡萄糖培养基分别在正常氧及缺氧培养条件培养,同时以5.5mmol/L葡萄糖培养基培养作为对照,氯化钴模拟缺氧环境,台盼兰染色检测细胞存活力,末端脱氧核苷酸转移酶介导的dUTP切口末端标记技术和AnnexinV-PI双染法流式细胞仪检测心肌细胞凋亡。结果正常氧条件下,33mmol/L葡萄糖时较5.5mmol/L葡萄糖时乳鼠心肌细胞存活率低(P<0.01),细胞凋亡率高(P<0.01);缺氧条件下,与5.5mmol/L葡萄糖浓度时比较,33mmol/L葡萄糖浓度时,心肌细胞存活率增高(P<0.01),凋亡率降低(P<0.05)。结论在正常氧条件下,高葡萄糖诱导乳鼠心肌细胞凋亡。氯化钴模拟缺氧条件下,高葡萄糖在乳鼠心肌细胞凋亡损伤中发挥保护作用。

Different Effects of Hyperglycemia on Normoxia and Hypoxia Cardiomycyte Apoptosis

Aim To investigate the effects of hyperglycemia on normoxia and hypoxia apoptosis in primary neonatal rat cardiac myocytes. Methods To culture the primary neonatal rat cardiac myocytes and exposure to normoxia and hypoxia environment in medium containing 33 mmol/L glucose and 5.5 mmol/L glucose (control). The hypoxia environment was achieved by treating cells with cobalt chloride. Cell viability was measured by Trypan blue staining. Cardiomyocyte apoptosis was detected with terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling and Annexin V/PI binding assay. Results In 33 mmol/L glucose group cell survival rate was lower (P<0.01) and apoptosic rate was higher (P<0.01) than those in 5.5 mmol/L glucose group in normoxia condition. However, in hypoxia condition, cell survival rate was higher (P<0.01) and apoptosic rate was lower (P<0.05) in 33 mmol/L glucose group than those in 5.5 mmol/L glucose group. Conclusion In cultured primary neonatal rat cardiac myocytes, hyperglycemia induced apoptosis in normxia condition. However, hyperglycemia plays an anti-apoptotic protective role in cobalt chloride mimic chemical hypoxia condition.