活化的蛋白激酶C受体1在非小细胞肺癌中的表达及意义

目的研究活化的蛋白激酶C受体1(RACK1)在非小细胞肺癌(NSCLC)中的表达及意义。方法采用免疫组织化学EnVision法检测RACK1及甲状腺转录因子-1(TTF-1)蛋白在123例NSCLC(包括组织学标本80份和胸腔积液细胞学标本43份)和50份正常肺组织中的表达情况。结果①RACK1和TTF-1蛋白在NSCLC癌组织中的阳性率分别为48.8%和42.5%,与正常肺组织阳性率4.0%和100.0%相比,差异均具有统计学意义(P<0.05)。在癌组织学标本中,RACK1和TTF-1蛋白在肺腺癌中的阳性率分别为87.5%和80.0%。在细胞学标本中,RACK1和TTF-1蛋白在肺腺癌中的阳性率分别为94.1%和85.3%。RACK1和TTF-1在肺腺癌中的表达率明显高于鳞癌和其他类型,其表达与组织学类型相关(P<0.05)。②RACK1蛋白诊断肺腺癌的灵敏度和特异度分别为90.5%和89.8%,TTF-1的灵敏度和特异度分别82.4%和95.9%。③在40例肺腺癌组织中,RACK1蛋白的表达与吸烟情况、TNM分期及淋巴结转移有关(P<0.05)。结论 RACK1促进了肺腺癌的发生、发展,其高表达可能提示患者预后不良,可能是较TTF-1更为敏感的肺腺癌诊断标记。

Expression and significance of receptor of activated kinase 1 in non-small cell lung carcinoma

Objective To investigate the expression and significance of the receptor of activated kinase 1 （RACK1） in non-small cell lung cancer （NSCLC）. Methods The expression of RACK1 and TFF-1 protein in 123 patients with NSCLC （including 80 histological samples and 43 pleural effusion cytology specimens） and 50 cases of normal lung tissue were examined by immunohistochemistry assay （EnVision technique）. Results The positive rates of RACKI and TYF-1 in NSCLC tissues （48.8%, 42.5%） were significantly different from that in normal lung tissues （4.0%, 100.0%） （both P〈0.05）. The positive expression rates of RACK1 and TTF-1 were 87.5% and 80.0% in lung adenocareinoma histological samples and 94.1% and 85.3% in the pleural effusion due to lung adenocarcinoma, re- spectively. The expression rates of RACK1 and TFF-1 were significantly higher than in squamous cell carcinoma and other types of lung adenocarcinoma （all P〈0.05）. The assay sensitivity and specificity of RACK1 and TTF-1 for the di- agnosis of lung adenoearcinoma were 90.5% and 82.4% as well as 89.8% and 95.9%, respectively. The expression of RACK1 was correlated with the smoking status, TNM stages and lymph metastasis （all P〈0.05） in 40 samples of lung adenocarcinoma. Conclusion RACK1 promotes the oncogenesis and development of lung adenocarcinoma and could be a crucial marker that was more sensitive than TYF-1 indicating a poorer prognosis.