miR‐30b‐5p up‐regulation related to the dismal prognosis for patients with renal cell cancer |
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Authors: | Chunduo Zhang Xiang Pan Xiqi Peng Kaihao Liu Jingyao Wang Liwen Zhao Xuan Chen Guocheng Huang Hang Li Jing Ye Yongqing Lai |
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Affiliation: | 1. Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen China ; 2. Department of Urology, Affiliated Hospital of Yangzhou University, Yangzhou China ; 3. Shantou University Medical College, Guangdong Shantou China ; 4. Anhui Medical University, Hefei China |
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Abstract: | The diagnosis of renal cell carcinoma (RCC) is often made late since there is no early symptom, which thus results in dismal patient prognosis. As a result, new biomarkers are urgently needed and efforts should be made to identify their functions in predicting RCC prognosis. microRNAs (miRNAs) are a class of small noncoding RNAs that are about 20‐22 nucleotides in length, and they have been demonstrated to function as prognostic markers in numerous tumors. This study aimed to assess the role of miR‐30b‐5p in predicting the prognosis of RCC postoperatively. In this study, RNA was extracted from 284 formalin‐fixed and paraffin‐embedded kidney cancer tissue samples. After cDNA synthesis, real‐time quantitative PCR (RT‐qPCR) was adopted for detecting the relative miR‐30b‐5p level. Then, the Kaplan‐Meier method, Cox regression analysis, and the receiver operating characteristic curve analysis were applied in analyzing the miR‐30b‐5p effect on the prognosis for patients. Our findings indicated that, following adjustment for age, gender, tumor stage, and tumor size, patients with low miR‐30b‐5p expression had remarkably longer overall survival. Thus, the miR‐30b‐5p level might be related to RCC prognosis. |
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Keywords: | miR‐ 30b‐ 5p, miRNAs, prognostic biomarkers, renal cell carcinoma |
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