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| 雌激素对大鼠血管平滑肌细胞囊泡素-1基因表达的影响 | |
| 引用本文： | 杨发林,刘贤锡,贺红,张建业,许伟华,张岩. 雌激素对大鼠血管平滑肌细胞囊泡素-1基因表达的影响[J]. 中国病理生理杂志, 2004, 20(9): 1638-1641 |
| 作者姓名： | 杨发林 刘贤锡 贺红 张建业 许伟华 张岩 |
| 作者单位： | 1. 山东大学医学院生物化学和分子生物学研究所,齐鲁医院, 山东 济南 250012; 2. 山东大学检验科, 山东 济南 250012; 3. 山东大学心内科, 山东 济南 250012; 4. 山东大学第二医院内科, 山东 济南 250012 |
| 摘 要： | 目的:探讨雌激素对血管平滑肌细胞囊泡素-1基因表达的影响。方法:取Wistar雌性大鼠,分为3组:假手术组,卵巢切除后皮下埋植雌激素组 (OVX+E组)及卵巢切除后皮下埋植安慰剂组(OVX+V组)。用药2周后处死大鼠,剥离主动脉平滑肌组织,提取总RNA进行半定量RT-PCR分析,检测雌激素对囊泡素-1(caveolin-1)基因表达的影响。为进一步明确雌激素是否直接调节血管平滑肌细胞caveolin-1基因表达,又采用100 nmol/L 17β-雌二醇(17β-E2)处理培养的大鼠血管平滑肌细胞24 h,通过Northern blot分析检测雌激素对细胞caveolin-1 mRNA表达的影响。结果:OVX+E组大鼠主动脉平滑肌组织caveolin-1基因表达量明显高于OVX+V组,17β-E2处理的培养细胞中caveolin-1基因mRNA表达量高于未用药的培养细胞。 结论:雌激素可促进血管平滑肌细胞caveolin-1基因表达,反映了雌激素心血管作用机理的一个方面。 |
| 关 键 词： | 雌激素 细胞质膜微囊蛋白 血管平滑肌细胞 |
| 文章编号： | 1000-4718(2004)09-1638-04 |
| 收稿时间： | 2003-12-23 |
| 修稿时间： | 2004-03-16 |
Effect of estrogen on the gene expression of caveolin- 1 in rat vascular smooth muscle cells |
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| YANG Fa-lin,LIU Xian-xi,HE Hong,ZHANG Jian-ye,XU Wei-hua,ZHANG Yan. Effect of estrogen on the gene expression of caveolin- 1 in rat vascular smooth muscle cells[J]. Chinese Journal of Pathophysiology, 2004, 20(9): 1638-1641 | |
| Authors: | YANG Fa-lin LIU Xian-xi HE Hong ZHANG Jian-ye XU Wei-hua ZHANG Yan |
| Affiliation: | 1. Institute of Biochemistry and Molecular Biology, Medical College, The Second Hospital, Shandong University, Jinan 250012, China; 2. Department of Clinical Laboratory, The Second Hospital, Shandong University, Jinan 250012, China; 3. Department of Cardiology, Qilu Hospital, The Second Hospital, Shandong University, Jinan 250012, China; 4. Department of Internal Medicine, The Second Hospital, Shandong University, Jinan 250012, China |
| Abstract: | AIM: To assess the effect of estrogen on the gene expression of caveolin-1 in rat vascular smooth muscle cells (VSMCs). METHODS: Wistar rats were ovariectomized and subjected to subcutaneous implantation of placebo pellets (OVX+V group) or estradiol pellets (OVX+E group). 2 weeks after implantation, the expression of caveolin-1 gene in endothelium-denuded aortic tissue was examined by RT-PCR. Furthermore, Northern blotting was used to analyze the mRNA expression of caveolin-1 in cultured rat VSMCs. RESULTS: RT-PCR showed that expression of caveolin-1 gene was significantly higher in OVX+E group than that in OVX+V group. Northern blot analysis showed that the mRNA expression of caveolin-1 was higher in VSMCs pretreated with 17β-estradiol (17β-E2) than that in VSMCs without 17β-E2 pretreatment. CONCLUSION: Estrogen up-regulates the gene expression of caveolin-1 in the vascular wall, partially indicating the cardiovascular effect of estrogen. |
| Keywords: | Estrogen Caveolins Vascular smooth muscle cells |
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