| 芥子碱对大鼠脑匀浆和血清中乙酰胆碱酯酶的抑制作用 |
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| 引用本文： | 何玲,李海涛,郭胜伟,刘丽芳,邱嘉斌,李富,蔡宝昌.芥子碱对大鼠脑匀浆和血清中乙酰胆碱酯酶的抑制作用[J].中国中药杂志,2008,33(7):813-815. |
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| 作者姓名： | 何玲 李海涛 郭胜伟 刘丽芳 邱嘉斌 李富 蔡宝昌 |
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| 作者单位： | 何玲 (南京中医药大学,博士后科研流动站,江苏,南京,210029中国药科大学,江苏,南京,210009)； 李海涛 (南京中医药大学,博士后科研流动站,江苏,南京,210029)； 郭胜伟 (南京中医药大学,博士后科研流动站,江苏,南京,210029)； 刘丽芳 (中国药科大学,江苏,南京,210009)； 邱嘉斌 (中国药科大学,江苏,南京,210009)； 李富 (中国药科大学,江苏,南京,210009)； 蔡宝昌 (南京中医药大学,博士后科研流动站,江苏,南京,210029)； |
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| 基金项目： | 中国博士后科学基金面上项目，江苏省博士后科学基金，江苏省药效研究平台项目 |
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| 摘 要： | 目的:考察中药单体芥子碱(3,5-二甲基,4-羟基苯丙烯酰胆碱,sinapine)对大鼠血清和大鼠大脑匀浆乙酰胆碱酯酶(acetylcholinesterase,AChE)的体外抑制作用。方法:大鼠眼球取血后断头,取大脑,匀浆,用双缩脲法测定脑组织匀浆总蛋白含量。采用乙酰胆碱脂酶试剂盒和改良Ellman比色法测定AChE的活力,检测芥子碱对大鼠血清和脑匀浆乙酰胆碱酯酶的抑制作用。结果:芥子碱明显抑制大鼠大脑匀浆AChE的活力,而对血浆AChE活性的抑制相对较弱,对脑匀浆和血清AChE的抑制IC50分别为3.66和22.1μmol.L-1,IC50血清/IC50脑=6.042(倍)。结论:芥子碱能显著抑制脑组织AChE活性,有可能作为中枢乙酰胆碱酯酶抑制剂(acholinesterase inhibi-tors,AChEIs)具有防治阿尔茨海默病(Alzheimer's disease,AD)的应用前景。
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| 关 键 词： | 芥子碱 乙酰胆碱酯酶 胆碱酯酶抑制剂 阿尔茨海默病 |
| 收稿时间： | 2007/10/11 0:00:00 |
Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats |
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| HE Ling ; LI Hai-tao ;GUO Sheng-wei ;LIU Li-fang ;QIU Jia-bin ;LI Fu ;CAI Bao-chang.Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats[J].China Journal of Chinese Materia Medica,2008,33(7):813-815. |
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| Authors: | HE Ling ; LI Hai-tao ;GUO Sheng-wei ;LIU Li-fang ;QIU Jia-bin ;LI Fu ;CAI Bao-chang |
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| Institution: | Postdoctoral scientific research afflux station, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China. |
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| Abstract: | OBJECTIVE: The present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterase (AChE) in cerebral homogenate and blood serum of rats. METHOD: AChE was prepared from cerebral homogenate and blood serum of rats, respectively. Acetylcholinesterase activity assay kit and Chromatometry were used to detect the AChE activity. RESULT: Sinapine significantly inhibited AChE activity in vitro, with more effective on cerebral homogenate (IC50 3.66 micromol x L(-1)) than blood serum (IC50 22.1 micromol x L(-1)). CONCLUSION: Sinapine could significantly inhibit the cerebral AChE activity and may be a promising drug used for prevention and cure of Alzheimer's disease as a cholinesterase inhibitor. |
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| Keywords: | sinapine acetylcholinesterase cholinesterase inhibitors Alzheimer's disease |
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