缺血性脑血管病患者血浆甘露糖结合凝集素检测及其启动子区多态性分析

目的：探讨甘露糖结合凝集素（MBL）血浆含量和存在形式以及启动子区多态性与缺血性脑血管病（ICVD）的关联性。方法：采集81例ICVD患者（ICVD组）和60例健康人群（对照组）抗凝血，ELISA法检测血浆MBL含量，Western blotting法检测血浆MBL寡聚体，SSP-PCR分析MBL启动子区－550（H/L）、－221（X/Y）和+4（P/Q）位点多态性。结果： ICVD组患者血浆MBL含量［(3 372.18±660.90) μg/L ］明显高于对照组［(2 065.29±195.67) μg/L］ (P<0.01)；ICVD组患者血浆MBL蛋白单体（约36 000）及寡聚体（约130 000和250 000）表达高于对照组；ICVD组患者与对照组MBL启动子单倍型和基因型构成比均不同，ICVD组患者启动子单倍型HYP（50.62%）和LXP（6.79%）构成比与对照组HYP（34.17%）和LXP（20.83%）构成比比较差异均有统计学意义（P<0.05）。基因型HXP/HYP和 HXP/LYQ仅见于ICVD组，HXP/LXP 和HYP/LXP则仅见于对照组； ICVD组LYQ/LYQ型频数（4.94%）显著低于对照组（15.00%）（P<0.05）。结论：ICVD患者血浆MBL含量及存在形式、启动子单倍型和基因型构成比均与健康人群不同，MBL可能参与了ICVD的发生、发展过程。

Detection of mannose binding lectin in plasma and analysis of its promoter region polymorphisms in patients with ischemic cerebrovascular disease

Objective To investigate the relevance between plasma content,plasma protein molecular forms and promoter region polymorphisms of mannose binding lectin(MBL) and ischemic cerebrovascular disease(ICVD).Methods The anti-coagulated blood of patients with ICVD(ICVD group,81 cases) and healthy people(control group,60 cases) was collected.The plasma level of MBL was measured by ELISA and the plasma MBL protein oligomeric forms were determined by Western blotting.The promoter region polymorphisms of MBL gene were analyzed by sequence specific primers PCR(SSP-PCR).Results The plasma content of MBL in ICVD group(3 372.18 μg·L-1±660.90 μg·L-1) was higher than that in control group(2 065.29 μg·L-1±195.67 μg·L-1)(P<0.01).The bands represented single MBL peptide sub-unit(approximately 36 000) and oligomeric forms(approximately 130 000 and 250 000) were deeper in ICVD group than those in control group.The constituent ratios of haplotype and genotype of MBL promoter region in ICVD group were significantly different from those in control group.There were significant differences in the frequencies of haplotype HYP and LXP between ICVD patients(50.62%,6.79%) and controls(34.17%,20.83%)(P<0.05).HXP/HYP and HXP/LYQ genotypes were found only in ICVD patients,meanwhile,HXP/LXP and HYP/LXP genotypes only in controls.ICVD patients had significantly lower frequency of genotype LYQ/LYQ(4.94%) compared with controls(15.00%)(P<0.05).Conclusion The plasma content,plasma protein molecular forms and constituent ratios of haplotype and genotype of MBL promoter region in ICVD patients are all different from those in healthy people,so MBL may be involved in the development and progression of ICVD.