IL－10对佐剂性关节炎大鼠腹腔巨噬细胞及脾淋巴细胞诱生IL－8的影响

本文研究了ＩＬ－１０对佐剂性关节炎（ＡＡ）大鼠腹腔巨噬细胞（Ｍφ）及脾淋巴细胞在体外诱生ＩＬ－８的影响，还探讨了ＩＬ－１０经腹腔注射后对ＡＡ大鼠的治疗作用以及在体内对ＩＬ－８合成的影响，并利用核酸杂交技术初步探讨了ＩＬ－１０的作用机制。结果表明，ＡＡ大鼠腹腔Ｍφ及脾淋巴细胞分泌ＩＬ－８的能力明显高于正常大鼠。在体外ＩＬ－１０能显著下调ＩＬ－８的表达。ＡＡ大鼠经ＩＬ－１０治疗后症状明显改善，ＩＬ－８的合成能力降低。ＩＬ－１０能明显下调正常大鼠腹腔ＭφＩＬ－８ｍＲＮＡ表达。总之，ＩＬ－１０在体内外均显著抑制ＩＬ－８的合成，并具有良好的抗炎活性，从而为临床治疗类风湿关节炎（ＲＡ）提供了新制剂。

Effects of IL-10 on the Secretion of IL-8 by Peritoneal Macrophagesand Splenic Lymphocytes in AA Rats

In this paper,we studied the effects of IL-10 on IL-8 production from peritonealmacrophages and splenic lymphocytes in AA rats,as well as its therapeutic effect onAA rats by peritoneal injection.The mechanism of action was investigaled preliminarily with molecular hybridization.Experimental results showed that peritoneal macrophages and splenic lymphocytes in AA rats could secrete more substantialIL-8 than that in normal's.IL-10 could significantly inhibited IL-8 production in peritoneal macrophages and splenic lymphocytes. IL-10 also ameliorated AA symptomsin rats. The ability to synthesize IL-8 was evidently decreased after treatment.IL-10 could dramatically down-regulated IL-8 mRNA expression from peritoneal macrophages in normal rats.In conclusion,IL-10 could significantly inhibit IL-8 production in vitro and in vivo with effective anti-inflammatory activity,thus providing anew agent for treatment of human rheumatoid arthritis(RA).