MNNG所致大鼠腺胃癌形成过程中机体自然抗癌机制的研究

目的　探讨机体的自然抗肿瘤作用。方法　给断乳雄性Wistar大鼠MNNG灌胃 ,每天一次 ,连续 10天 ,以诱导大鼠腺胃粘膜异倍体形成 (大鼠腺胃癌模型形成 )。第 2 5周时 ,用流式细胞仪测定大鼠腺胃幽门粘膜异倍体形成的情况 ,用免疫组织化学ABC法观察了大鼠腺胃粘膜异倍体形成过程中 ,腺胃胃泌素细胞胃泌素细胞、生长抑素细胞、5-羟色胺细胞的免疫组织化学变化 ,并对以上结果进行定性、定位、定量分析以及统计学处理。结果　在MNNG所致大鼠腺胃癌形成过程中其腺胃胃泌素细胞、生长抑素细胞、5-羟色胺细胞的免疫组织化学阳性强度均明显增强 (P <0 .0 1,P <0 .0 5)。结论　胃内分泌细胞可能介导了MNNG所致大鼠胃癌的发生和发展 ;有关机理尚待进一步探讨。

Study on a Natural Carcinogenesis-Resistant in the Course of Rat Glandular Stomach Carcinogenesis

Objective To investigate the mechanism of a natural carcinogenesis-resistant. Methods In our experiment, Wistar rat stomach carcinogenesis (formation of aneuploid cells in the glandular stomach mucosa) was induced by MNNG 20mg/kg gavaged, once a day for 10 days. At the 25th week,the incidence of aneuploid cells in mucosal epithelium of gastric antrum was determined by flow cytometry (FCM). Changes of Gastrin cell, Somatostatin cell in Wistar rat glandular stomach were detected by ABC immunohistochemical method in the course of selenium preventing formation of aneuploid cells. Situation and quantitative analyses of the results were made by image analysis, and statistical analysis was made by SPSS ware. Results It was significantly increased in immunohistochemical activities of Wistar rat glandular stomach Gastrin cells, somatostatin cells and 5-hydroxytryptamine cells in the course of the rat stomach carcinogenesis(P<0.01,P<0.05). Conclusion This study reveals rat stomach carcinogenesis induced by MNNG might be introduced by endocrine cell of rat stomach. The mechanism of relationship needs to be further explored.