| 应用酵母双杂交研究G-CSF受体胞内区结合蛋白 |
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| 引用本文： | 苑晓玲,从玉文,郝静,李冬严,善亚君,王金惠,赵振虎,陈家佩. 应用酵母双杂交研究G-CSF受体胞内区结合蛋白[J]. 军事医学科学院院刊, 2002, 26(3): 185-187,190 |
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| 作者姓名： | 苑晓玲 从玉文 郝静 李冬严 善亚君 王金惠 赵振虎 陈家佩 |
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| 作者单位： | 军事医学科学院放射医学研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金 (3 0 10 0 0 61)资助 |
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| 摘 要： | 目的：探寻与G-CSF受体胞内区相互作用的下游蛋白因子，揭示G-CSF受体信号转导通路的可能机制。方法：采用敏感性较高的酵母双杂交体系，筛选小鼠胎肝文库，并通过体内双杂交实验进行验证。结果：获得了11种阳性基因片段，对其中铁蛋白重链片段在体内验证了相互作用。结论：提示在小鼠胎肝中G-CSF受体作为胞内信号转导的起始点，与众多胞浆内蛋白因子相结合，对于揭示G-CSF受体信号转导可能存在的各种通路起到重要的提示作用。
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| 关 键 词： | 粒细胞集落刺激因子受体 酵母双杂交系统 信号传递 |
| 文章编号： | 1000-5501(2002)03-0185-04 |
Hunting for novel proteins in intracellular G-CSFR with yeast two-hybrid system |
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| YUAN Xiao_Ling,CONG Yu_Wen,HAO Jing,LI Dong_Yan,SHAN Ya_Jun,WANG Jin_Hui,ZHAO Zhenhu,CHEN Jia_Pei. Hunting for novel proteins in intracellular G-CSFR with yeast two-hybrid system[J]. Bulletin of the Academy of Military Medical Sciences, 2002, 26(3): 185-187,190 |
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| Authors: | YUAN Xiao_Ling CONG Yu_Wen HAO Jing LI Dong_Yan SHAN Ya_Jun WANG Jin_Hui ZHAO Zhenhu CHEN Jia_Pei |
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| Abstract: | Objective:To explore the protein factors that may interact with G_CSF receptor in the signal transduction pathways.Methods:The highly sensitive protein_protein interaction system--yeast two_hybrid system was applied to screen the mouse fetal liver library. The interaction of GST_ferritin with His_GCSFR in vitro was examined with mammalian two_hybrid expriment.Results:Eleven positive gene fragments were obtained,and mammalian two_hybrid experiment confirmed that ferritin heavy chain interacted with intracellular G_CSF receptor.Conclusions:It is the first time to find that in mouse fetal liver tissue, G_CSF receptor can interact with many protein factors to activate some signaling pathways. This result may be important for understanding the relationship between protein factors and G_CSF receptor. |
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| Keywords: | G_CSF receptor yeast two_hybrid system signal transduction |
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