补肾活血汤对小鼠体外受精及其早期胚胎发育的影响

目的探讨补肾活血汤对女性生殖活动的影响，揭示其治疗不孕的作用机理。方法以补肾活血汤的兔含药血清作为培养基添加剂，分别与未交配小鼠的精、卵及交配后雌鼠体内回收的2细胞期胚胎共同体外培养，观察小鼠体外受精及早期胚胎体外发育的变化。结果含药血清加入培养能显著提高小鼠的体外受精率(P＜0.01)，并可促进其后的各期胚胎发育，尤其对4细胞及8细胞期胚胎的发育有显著促进作用(P＜0.05)；对小鼠体内2细胞期胚胎的体外4细胞发育率无明显影响，而对其8细胞期胚、桑椹胚、囊胚、孵化胚的各期发育率均有明显促进作用(P＜0.05或P＜0.01)。结论补肾活血汤具有提高卵子受精率和促进早期胚胎发育的作用。

Effect of Bushen Huoxue Decoction on in vitro Fertilization and Early Embryonic Development in Mice

To explore the effect of Bushen Huoxue decoction (BSHX) on female reproduction and elucidate its therapeutic mechanism to infertility. Methods: The BSHX medicated serum of rabbit, as a supplement, was co cultured with the sperm and ovum of non copulated mice, and the 2 cell embryos of copulated female mice separately, to observe the changes of in vitro fertilization rate (IVF) and early embryogenesis rate. Results: By co cultured with BSHX medicated serum, IVF rate was increased obviously (P<0 01), and the follow up early embryogenesis rate at various period was promoted, particularly that of the 4 cell and 8 cell embryos (P<0 05). No influence on developmental rate of in vitro 4 cell embryos obtained from the in vivo 2 cell embryos. But the development of the 8 cell embryos, morula, blastula and hatching were promoted significantly. Conclusion: BSHX could raise the fertilization rate and promote the early embryonic development.