吸烟对精子DNA完整性、精子参数的影响

目的:探讨吸烟对精子DNA完整性、精子参数的影响。方法:调查191例男性不育患者的吸烟情况,采用吖啶橙荧光染色后检测精子DNA完整性,计算机自动分析精子密度与活力,精子形态检测系统下人工修正方法进行精子形态分析。结果:吸烟组精子活力显著低于不吸烟组(P<0.05),精子密度和形态低于不吸烟组,但差异无统计学意义(P>0.05)。吸烟组精子DNA完整率异常例数显著高于不吸烟组(χ2=5.393,P<0.05),精子DNA完整率异常组精子密度显著低于精子DNA完整率正常组(P<0.05),精子DNA完整率异常组的精子活力、形态与正常组相比差异无统计学意义(P>0.05)。结论:吸烟影响精子DNA完整率;精子DNA完整率异常与精子参数异常相关;吸烟可能通过影响精子DNA完整性影响精子参数。

Effect of cigarette smoking on human sperm DNA integrity and sperm parameters

Objective:To explore the effect of cigarette smoking on human sperm DNA integrity and sperm parameters.Methods:The cigarette smoking status of 191 patients with male infertility were surveyed,human sperm DNA integrity was detected by fluorescent staining of acridine orange.Sperm density and motility were detected by computer assisted sperm analysis.Sperm morphology analysis was performed by semiautomated sperm morphology analyzer.Results:Sperm motility of smoker group was significantly lower than that of nonsmoker group(P<0.05),the sperm density and morphology were lower than those of nonsmoker group,but without significant difference(P>0.05).Abnormal cases of sperm DNA integrity of smoker group was significantly higher than that of nonsmoker group(χ2=5.393,P<0.05).Sperm density of abnormal sperm DNA integrity group was significantly lower than that of normal group(P<0.05),but there was no difference in sperm motility and morphology between the two groups.Conclusion:Cigarette smoking affects sperm DNA integrity.There is a correlation between sperm DNA integrity and sperm density.Cigarette smoking may affect on sperm parameter by sperm DNA integrity.