参附注射液对新生大鼠缺氧缺血性脑损伤的干预作用

目的:研究参附注射液对新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的干预作用。方法:建立新生大鼠HIBD模型,应用HE染色、Annexin V/PI染色法、免疫组化染色法观察正常对照组、假手术组、生理盐水组及参附干预组在缺氧缺血后2 h、12 h、24 h、3天、7天、14天的病理变化、神经细胞凋亡及Bax、Bcl-2蛋白表达。结果:①缺氧缺血后生理盐水组海马CA1区病理变化明显,参附干预组上述变化减轻。②正常对照组及假手术组海马CA1区Bcl-2表达较弱,手术后该区Bcl-2表达仍弱,Bax表达增强,出现凋亡细胞,以24 h最明显;参附干预组Bcl-2表达较生理盐水组增强,而Bax表达减弱(P<0.05),凋亡细胞减少。结论:HIBD后Bax蛋白表达增强,细胞凋亡增加。参附可上调Bcl-2表达,下调Bax表达,使凋亡细胞减少,这可能是参附注射液治疗新生儿缺氧缺血性脑损伤的机制之一。

Effect of Shenfu injection on hypoxic-ischemic brain damage of neonatal rats

Objective:To investigate the effect of Shenfu injection on neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods:After the establishment of HIBD model in neonatal rats,the pathologic changes,nerve cell apoptosis and the expression levels of Bcl-2 and Bax were observed in control group,pseudo-operation group,saline group and Shenfu intervention group at 2,12 and 24 hours,3,7 and 14 days by HE staining,Annexin V/PI staining and immunohistochemical staining.Results:①The pathological changes of the hippocampal CA1 region in saline group was more apparent than those in Shenfu intervention group.②There was no difference in the expression level of Bcl-2 in the CA1 region before and after operation in control group and pseudo-operation group,but the expression level of Bax increased after operation and reached the peak at 24 hours.The expression of Bcl-2 in Shenfu intervention group was higher than that in saline group,but the expression level of Bax in Shenfu intervention group was lower than that in saline group(P<0.05),the amount of apoptotic cells decreased.Conclusion:The expression level of Bax increases after HIBD,Shenfu up-regulates Bcl-2 expression and down-regulates Bax expression,which maybe the possible therapeutic mechanism of Shenfu on the brain after hypoxic-ischemic damage in neonates.