孕期及哺乳期低剂量三丁基锡暴露对子代小鼠生长发育的影响

目的 探讨孕期及哺乳期低剂量三丁基锡(TBT)暴露对子代昆明(KM)小鼠生长发育的影响.方法 将清洁级健康成年妊娠昆明小鼠随机分为100、10、1μg/kg TBT染毒组和阴性对照组(玉米油),每组6只.从妊娠第6天(GD6)起,采用经口灌胃方式进行染毒,每天1次,直至哺乳期结束,灌胃剂量按照每天称量的体重计算.仔鼠出生后记录仔鼠数量,雌、雄数量及体重,仔鼠出生后第1、3、5、7、14、21天时称量仔鼠体重,出生后第12天(PND12)开始观察仔鼠睁眼情况,出生后第13天开始观察睾丸下降情况,出生后第20天开始观察阴道张开情况.结果 100μg/kg剂量组母鼠孕期体重净增加量显著降低(P＜0.01),各染毒组母鼠哺乳期体重增加量、产仔数量及仔鼠雌、雄比例,差异无统计学意义(P＞0.05).10μg/kg剂量组仔鼠体重在出生后第1天和各染毒组仔鼠在出生后第3、5、21天比对照组显著降低(P＜0.05,P＜0.01),100μg/kg剂量组仔鼠出生后第1天到第3天体重增加量及10μg,kg和100μg/kg剂量组仔鼠在出生后第14到20天体重增加最比对照组显著降低(P＜0.05,P＜0.01).各TBT染毒组仔鼠的阴道张开时间比对照组显著提前,睁眼时间、睾丸下降时间显著延迟(P＜0.05,P＜0.01)且随着染毒剂量的增加睁眼时间有延迟的趋势.结论 孕期及哺乳期低剂量TBT暴露可以影响仔鼠的生长发育.

Abstract：

Objective To explore the effects of gestation and lactation exposure to low dose tributyltin (TBT)on development of offspring of Kunming mice.Methods The healthy adult pregnant Kunming mice were randomly divided into 4 groups,6 in each group,they were given doses of TBT(100,10 and 1 μg/kg)by gavage from days 6 of gestation(GD6)to the end of lactation.The number of pups and the number of male,female of each dams were determined,and the body weight of pups on PNDs1,3,5,7,14,21 was weighed.The eye opening from PND12 and the testes descent from PND13 and the vagina opening from PND20were examined.Results Compared with the control group,the gestational body weight gain of dams decreased in 100μg/kg group(P＜0.01).The lactational body weight gain,the number of pups and ratio of female and male were no significant difference among groups.The time of eye opening was significantly delayed and the vagina opening was significantly ahead in treatment groups(P＜0.05.P＜0.01),and there was a increasing trend in time of eye opening following the TBT dose rising.Conclusion Gestation and lactation exposure to low-dose TBT may have adverse effects on the growth and development of offspring of Kunming mice.

Effect of Gestation and Lactation Exposure to Low-dose Tributyltin on Development of Mice Offspring

Objective To explore the effects of gestation and lactation exposure to low dose tributyltin (TBT)on development of offspring of Kunming mice.Methods The healthy adult pregnant Kunming mice were randomly divided into 4 groups,6 in each group,they were given doses of TBT(100,10 and 1 μg/kg)by gavage from days 6 of gestation(GD6)to the end of lactation.The number of pups and the number of male,female of each dams were determined,and the body weight of pups on PNDs1,3,5,7,14,21 was weighed.The eye opening from PND12 and the testes descent from PND13 and the vagina opening from PND20were examined.Results Compared with the control group,the gestational body weight gain of dams decreased in 100μg/kg group(P＜0.01).The lactational body weight gain,the number of pups and ratio of female and male were no significant difference among groups.The time of eye opening was significantly delayed and the vagina opening was significantly ahead in treatment groups(P＜0.05.P＜0.01),and there was a increasing trend in time of eye opening following the TBT dose rising.Conclusion Gestation and lactation exposure to low-dose TBT may have adverse effects on the growth and development of offspring of Kunming mice.