孕中期应用金标法和酶联免疫法筛查唐氏综合征9554例分析

目的:通过采用不同方法筛查唐氏综合征(DS),以选择利于在条件有限的基层医院开展产前筛查。方法:采用金标法半定量快速筛查,判读结果;采用酶联免疫法检测孕妇血清甲胎蛋白(AFP)和游离β人绒毛膜促性腺激素的含量(Free-β-hCG),进行DS风险率计算。结果:接受筛查的孕妇9554例,其中采用金标法筛查4197例,DS筛查阳性孕妇243例,占筛查总数的5.79%,有47例接受羊水产前诊断,确诊为DS 5例,其他染色体异常7例;采用酶联免疫法筛查5357例,DS筛查为高风险孕妇444例,占筛查总数的8.29%,有207例接受羊水产前诊断,确诊胎儿为DS 8例,其他染色体异常26例。结论:金标法是一种有效的产前筛查方法,可用于一些出生率低、条件有限的中小型医院,对在基层卫生组织广泛开展与普及产前筛查工作有积极作用。

Analysis on Down syndrome screening by dot immunogold filtration assay and enzyme linked immunosorbent assay in 9 554 cases in second trimester

Objective:To evaluate the effectiveness of different methods in screening of Down syndrome,choose preferable method for prenatal screening in primary hospital.Methods:The levels of serum alpha fetoprotein and free β-hCG in pregnant women were detected by dot immunogold filtration assay and enzyme linked immunosorbent assay,and the risk ratio was calculated.Results:Among 9 554 cases,4 197 cases were detected by dot immunogold filtration assay,the positive rate was 5.79%(243 cases),47 cases received amniotic fluid examination,and 5 cases were diagnosed as Down syndrome,7 cases were diagnosed as chromosome abnormalities;5 357 cases were detected by enzyme linked immunosorbent assay,the high risk rate was 8.29%(444 cases),207 cases received amniotic fluid examination,and 8 fetuses were diagnosed as Down syndrome,26 cases were diagnosed as chromosome abnormalities.Conclusion:Dot immunogold filtration assay is an effective method in prenatal screening of Down syndrome,which can be used in primary hospitals.