广州管圆线虫感染大小鼠后脑内水通道蛋白4表达水平研究

目的研究水通道蛋白4（AQP-4）在广州管圆线虫感染大小鼠脑组织中的表达水平及其与脑水肿的关系。方法在不同感染时期（0d、7d、14d、21d），用ReahimePCR法检测大鼠感染不同时间组，及米诺环素给药＋感染组和单纯感染组小鼠脑组织中AQP-4的表达水平，测定各组小鼠脑组织含水量。结果大鼠感染广州管圆线虫后脑组织中AQP-4的表达水平在7d时有升高，而其他时间都无明显变化。小鼠感染广州管圆线虫后，脑组织中的AQP-4水平明显升高，且成持续升高状态。用米诺环素抑制小胶质细胞后，给药组小鼠脑内AQP-4的水平明显下降。脑组织含水量也低于单纯感染组。结论AQP-4与广州管圆线虫感染所致的小鼠脑水肿密切相关，小胶质细胞可能是参与调节这-过程的重要细胞。

Study on the expression level of AQP-4 in rats and mice brain infected by Angiostrongylus cantonensis

Objective To study the expression level of aquaporin-4 （AQP-4） in brain of rats and mice intected by Angiostrongylus cantonensis （A. cantonensis）, and clarify the relationship of AQP-4 and brain edema. Methods The mRNA level of AQP-4 in brain tissue of infected rats, infected mice and minocycline treated infected mice were detected by Real-time PCR after A. cantonensis infection 0 d,7 d, 14 d and 21 d. The water content of mice brain was also measured. Results AQP-4 mRNA level in rats brain increased after 7 days infection and did not rise at other time points. However, AQP-4 level increased continuously after infection in mice brain. When mice were treated by minoeycline, the mRNA level of AQP-4 decreased evidently. At the same time, the water content of minocycline treated mice brain tissue was reduced comparing with the untreated infected mice. Conclusion The high level of AQP-4 in mice brain was associated with brain edema which was induced by A. cantonensis, and microglia may regulate this progress.