| 甲胎蛋白、癌胚抗原时间分辨荧光法与化学发光法比较 |
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| 引用本文： | 王桂莲,朱利国,黄飚,蔡刚明,谭成,金坚. 甲胎蛋白、癌胚抗原时间分辨荧光法与化学发光法比较[J]. 南通医学院学报, 2000, 20(2): 159-159,61 |
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| 作者姓名： | 王桂莲 朱利国 黄飚 蔡刚明 谭成 金坚 |
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| 作者单位： | 江苏省江原医院,无锡,241063 |
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| 摘 要： | ＤＴＰＡ法制备了Ｅｕ＾３＋标单抗,采用夹心法建立了ＡＦＰ和ＣＥＡ ＩＦＭＡ。它们的灵敏度分别为４３ｎｇ／Ｌ和９０ｎｇ／Ｌ,批内ＣＶ为１．５９９％和１．３６％,批间ＣＶ为７．１３９％和２．８９％,平均回收率为１０３．９８％和９２．７８％。与ＣＬＩＡ进行临床应用比较显示结果高度相关（ＡＦＰ ｒ＝０．９０４０,ＣＥＡ ｒ＝０．９７０５）。
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| 关 键 词： | 甲胎蛋白 癌胚抗原 肿瘤 诊断 时间分辨荧光法 |
EVALUATION OF TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY AND CHEMILUMINESCENCE IMMUNOASSAY FOR AFP AND CEA |
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| WANG Guilian,ZHU Liguo,HUANG Biao,et al. EVALUATION OF TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY AND CHEMILUMINESCENCE IMMUNOASSAY FOR AFP AND CEA[J]. ACTA Academiae Medicinae Nantong, 2000, 20(2): 159-159,61 |
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| Authors: | WANG Guilian ZHU Liguo HUANG Biao et al |
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| Abstract: | Two time-resolved Immunofluorometric assay (IFMA)of AFP and CEA were established with the two-sites "sandwich" method, by preparing Eu 3 -MocAb using the DTPA method. Their sensitivities were 43ng/L and 90ng/L,respectively.The recovery rates were 103.98% and 92.78%. The inter- and inter-assay CV were 1.36% and 2.89% for CEA IFMA,1.599% and 7.139% from AFP IFMA. Compared these methods with Chemiluminescence Immunoassay for CIBACORNING Co for patients, they were interrelated with each other, repression eoafficients was 0.9040 for measured AFP, 0.9705 for measured CEA on clinical analysis. [ |
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| Keywords: | time-resolved Immunofluorometric assay chemiluminescence Immunoassay AFP CEA |
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