高血压—糖尿病大鼠外周血淋巴细胞β受体动态变化的研究

采用放射配基结合法动态观察高血压—糖尿病(HD)大鼠在扩张型心肌病(DCM)病程中,外周血淋巴细胞β受体的变化。结果表明:HD大鼠3个月组血淋巴细胞β受体密度(Bmax)较对照组上升41.28%(P<0.01),平衡解离常数(Kd)则下降40.06%(P<0.05);6个月组Bmax较对照组上升119.55%(P<0.001),kd上升92.42%(P<0.002); 8个月组Bmax较对照组下降29.32%(P<0.001),Kd则上升88.38%(P<0.05),10个月组Bmax与对照组无明显差别(P>0.05),Kd则上升50.01%(P<0.05)。在病程中HD大鼠心肌β受体密度和血淋巴细胞β受体密度相关性良好(r=0.9545,t=4.122,P<0.05)。β受体的性质改变出现在心肌病理改变之前,β受体的下行调节和心肌病理改变的时相一致,提示血淋巴细胞β受体在DCM病程中从上行调节转为下行调节,可作为DCM时观察心肌β受体的“窗口”。

The changes of beta-adrenergic receptor on circulating intact lymphocytes in hypertensive-diabetic rats

Some kinetic characteristics of beta-adrenoceptor on circulating intact lymphocytes (CIL) in the combined renovasoeular hypertensive and diabetic (HD) rats were studied and the relation of betareceptos between CIL and cardium as well as the morphologic changes in the processe of dilated cardiomypathy (DCM) were observed. The results show that: the density of beta-adrenoceptor on CIL (Bmax) was going up 41.28%(P<0.01) and the dissociation constant(Kd) for [~3H] DHA was going up 40.6%(P<0.05) in 3rd month group in comparison with the control group, The Bmax and Kd of 6th month group sharply increased by 119.55%(P<0.001) and 92.42%(P<0.002), The Bmax of 8th month group was dramatically down about 29.3%(P<0.01), The Kd increased by 88.38%(P<0.02) at the same time. The Bmax in 10th month group had no singnificant difference in comparison with that in the controls (P>0.05), but the Kd was still 50.01%(P<0.05) higher. The relationship of the densities of beta-adrenoceptors between myocardium and CIL is fairly parallel(r=0.9495P<0.050). The changes of beta-adrenoceptors on CIL and heart happened before the structure alterations of myocardium. The down-regulation of beta-adrenoceptors of CIL and cardiac morphologic happened at the some time. The presented data suggested that the kinetic changes of betareceptors on CIL can be used as a good marker to reflect the changes of beta-adrenoceptors in myocardium during the process of DCM and could be potentially used in clinic practice.