葛根素治疗2型糖尿病KKA~y小鼠肝脏损伤的实验研究

目的 研究2型糖尿病（T2DM）肝脏损伤的发生及可能的发病机制，探讨葛根素注射液的干预作用。

Effect of Puerarin on Liver Injury in KKAy Mice with Type 2 Diabetes Mellitus

Objective To study the possible pathogenic mechanism of liver injury in type 2 diabetes mellitus （T2DM） and the intervening effect of puerarin on it. Methods Mice with T2DM （KKA^y） were randomly divided into two groups, the model group and the puerarin group. And the C57BL/J mice of the same age were set up as normal controls. They were sacrificed at 28 weeks old for observing serum fasting blood glucose （ FBG）, triglyceride （ TG）, total cholesterol （TC）, alanine aminotransferase （ ALT）, aspartate aminotransferase （AST） by automatic biochemistry; liver cell apoptosis by flow cytometry; pathomorphology by electron microscope; and mRNA expressions of bcl-2 and bax genes by RT-PCR; as well as the activities of superoxide dismutase （ SOD）, glutathione peroxidase （ GSH-Px）, Na ^＋-K^ ＋ -ATPase ; and content of malondialdehyde （MDA） in liver tissue by spectrophotometer. Results In KKA^y mice, blood levels of FBG, TG, TC, ALT, AST and liver cell apoptosis rate were higher; the bax mRNA expression was higher and bcl-2 mRNA was lower markedly; the activities of SOD, GSH-Px,Na^＋-K^＋-ATPase in liver tissue were lower, and MDA content was higher than those in the normal control significantly （ all P 〈 0.01 ）. Besides, mitochondria Swelling and damage were found in liver tissue. While in the puerarin group after treatment, all the above-mentioned changes were alleviated to some extent. Conclusions Obvious liver injury emerges in KKA^y mice. Puerarirl shows a protective effect on the T2DM caused oxidative damage by way of up-regulating bcl-2 to inhibit oxidative stress, and improving the energy metabolic dysfunction in liver of mice.