萃取-显色法快速测定莪术油脂质体包封率

目的：建立快速测定莪术油脂质体（ZTO-L）包封率的方法。方法：采用香草醛硫酸显色法,在波长520nm处测定脂质体中莪术醇的含量,利用石油醚萃取法分离ZTO-L与游离药物,考察石油醚用量与脂质体和药物回收率的关系;并与葡聚糖凝胶柱色谱分离法和透析法进行比较。结果：莪术醇浓度在0.8～6.0μg·ml-1线性关系良好（r=0.997）。莪术油中莪术醇的百分含量为70.37%。石油醚萃取法对空白脂质体的回收率为99.34%,对莪术醇回收率为98.77%,用该法所测脂质体平均包封率为70.78%。透析法不适合用于分离ZTO-L与游离药物。结论：萃取-显色法可快速准确地测定ZTO-L的包封率,且不受脂质体粒径、ZETA电位的限制。

Rapid determination of entrapment efficiency of zedoary turmeric oil lipsomes by extraction- chromogenic method

Objective：To establish conditions for entrapment efficiency rapid determination of zedoary turmeric oil lipsomes（ZTO-L）.Method： Vanillin-sulfuric acid chromogenic method was used to determine the concentration of curcumenol in ZTO-L at 520nm.Petroleum ether extraction method was used to separate the ZTO-L and free drug,and compared with glucan gel column chromatography and dialysis methods. Result： A linear relationship between the concentration of curcumenol and absorption was observed in the concentration rage of 0.8~6.0μg·ml-1;the precision was good（r=0.997）. The percentage of curcumenol in zedoary turmeric oil was 70.37%. For petroleum ether extraction method,the recovery rate of blank liposomes was 98.77%;the recovery rate of curcumenol was 98.77%. The entrapment efficiency of this lipsomes was 70.78% determined by petroleum ether extraction method.Dialysis method was not suitable to separate the ZTO-L and free drug. Conclusion： Extraction- chromogenic method is a fast and accurate method to separate the ZTO-L and free drug and not affected by partical size and ZETA potential of lipsomes.