小柴胡汤冲剂治疗子宫内膜异位症的实验研究

目的采用大鼠子宫内膜异位症(EMS)动物模型,探讨中药制剂小柴胡汤冲剂(XCHT)对EMS异位内膜的作用.方法通过透射电镜技术观察XCHT对异位内膜生长和细胞结构的影响.结果中、高剂量XCHT(10g/kg、15g/kg)组对异位内膜生长有明显抑制作用,异位内膜体积明显减小(P<0.01),低剂量XCHT(5g/kg)组体积也有减小(P<0.05),而对照组体积无明显减小(P>0.05).透射电镜下可见中、高剂量XCHT组治疗后较多腺上皮细胞出现凋亡的特征,表现为细胞体积变小、核固缩、胞浆和核染色质凝集,密度增高,细胞间凋亡小体,间质细胞中也可见一些坏死细胞.结论XCHT对EMS大鼠异位内膜有明显抑制作用,其作用机制可能是通过调整宿主局部环境中的免疫状态和功能,而诱导细胞的凋亡和坏死.

An Experimental Study on Xiaochaihu Decoction for Endometriosis in Rats

Objective To observe the effect of Xiaochaihu decoction(XCD) on experimental endometriosis in rats.Methods Rat models of endometriosis were established and the effect of XCD were evaluated by measuring the volume of ectopic endometrium (EE) and its ultrastructure with transmission electron microscopy.Results After treatment,moderate and high-dosage of XCD(10g/kg and 15g/kg respectively) could markedly inhibit endometrial explant growth and decrease the volume of EE(P < 0.01).Low-dosage of XCD could also decrease the volume of EE(P < 0.05),but the control group did not markedly decreased(P > 0.05).Meanwhile,the glandular cells in the endometrium after treatment in the moderate and high-dosage groups showed characteristic features of apoptosis:cellular atrophy,condensation of cytoplasm and nuclear chromatin and increase of their density,occurrence of the intercellular apoptotic bodies as well as degeneration and necrosis of stromal cell.Conclusion XCD has obvious effects in inhibiting ectopic endometrium and its mechanism may be related to the reduction of cell death (including apoptosis and necrosis) by regulating the immune function.