E-钙粘素、基质金属蛋白酶-2及其抑制剂与大肠癌浸润转移的关系

目的:探讨E-钙粘素(E-Cad)、基质金属蛋白酶-2(MMP-2)及其抑制剂(TIMP-2)表达与大肠癌浸润转移的关系。方法:采用S-P免疫组织化学染色技术,检测30例大肠腺癌,60例大肠癌组织E-Cad、MMP-2和TIMP-2的表达情况。结果:E-Cad的表达率在大肠腺瘤中为87.10%,显著高于大肠癌中的55.10%(P<0.05);其表达与大肠癌的大体类型有关,且随着大肠癌分化程度的降低而减少,与淋巴结转移呈负相关,E-Cad表达率越高患者的预后越好(P均<0.05)。MMP-2的表达率在大肠腺瘤中为26.67%,显著低于大肠癌中的86.67％(P<0.05);其表达与大肠癌的Dukes分期、分化程度、淋巴结转移和生存期均密切相关(P均<0.05)。TIMP-2的表达在大肠腺癌和大肠癌组织中没有显著性差异(P均>0.05),但其表达与大肠癌的Dukes分期、淋巴结转移、远隔脏器转移及生存期均有关(P均<0.05)。结论:E-Cad、MMP-2和TIMP-2的检测可以成为临床判断大肠癌的恶性程度、转移及预后的重要参考指标。

Relationship Between Expression of E-cadherin,Matrix Metaloproteinases-2, Tissue Inhibitor of Metalloproteinase-2 and the Invasion Metastasis of Colorectal Cancer

Puipose:To investigate the relationship between expression of E-cadherin(E-Cad),matrix metalloproteinases-2 (MMP-2) .tissue inhibitor of metalloproteinases-2( TIMP-2) and carcinmatosis,progression of colorectal cancer(CRC) .Methods:The E-Cad and MMP-2 expression was studied by im-munohistochemisry staining in 30 specimens of colorectal adenoma and 60 specimens of CRC. Results:The expression rates of E-Cad was 87.10% in colorectal adenoma and 55.00 % in CRC, the former was significant higher than the later( P < 0.05) . The expression of E-Cad had relation to growth style, and it was decreased along with the decreased of celluar dediffemtiation. The expression of E-Cad had negative correlation to metastasis of lymph node and had positive correlation to prognosis( P < 0.05). The expression rates of MMP-2 was 26.67 % in colorectal adenoma and 86.67 % in CRC, the later was significant higher than the former( P < 0.05). There were significant relationship observed between MMP-2 expression and Dukes stage, cellular dedifferen-tiation, metastasis of lymph node and prognosis (f<0.05).No significant difference in expression of TIMP-2 between colorectal adenoma and CRC(P> 0.05);but there were significant relationship between TIMP-2 expression and Dukes stage,metastasis of lymph node and distant organs, and prognosis (P < 0.05}. Conclusion: The detection of expression of E-Cad, MMP-2 and TIMP-2 may be helpful to judge malignant behavier, metastasis and prognosis in colorectal carcinoma.