腺苷对人肺腺癌细胞A549增殖和细胞周期的影响

目的：探讨腺苷对人肺腺癌细胞株A549增殖和细胞周期的影响。方法：体外培养肺腺癌细胞株A549,采用RT-PCR方法检测腺苷受体mRNA在A549细胞中的表达情况。以0、1、10、100和1 000μmol/L的腺苷分别作用A549细胞24、48和72 h后,采用MTT方法检测细胞光密度值,分析不同时间、不同浓度的腺苷对A549细胞存活率的影响。以100μmol/L的腺苷作用A549细胞24 h,采用流式细胞术检测其细胞周期。对上述所得数据进行单因素方差分析和独立样本t检验。结果：四种腺苷受体A1、A2A、A2B和A3在A549细胞中均有表达。MTT结果显示,作用时间24 h,各腺苷处理组（1、10、100和1 000μmol/L）细胞存活率分别为91%、84%、80%、73%;作用时间48 h,各腺苷处理组细胞存活率分别为82%、69%、58%、55%;作用时间72 h,各腺苷处理组细胞存活率分别为86%、56%、52%、38%,表明腺苷对A549细胞具有生长抑制效应,抑制率随着腺苷浓度升高而增强。与对照组相比,100μmol/L腺苷作用于A549细胞24 h后,能增加G1期细胞比例（对照组：59.07±0.70;100μmol/L：65.05±0.62,t=-11.04,P〈0.01）,并减少G2期细胞比例（对照组：6.67±0.95;100μmol/L：1.29±0.43,t=8.89,P〈0.01）。结论：腺苷能够抑制人肺腺癌细胞A549的生长,并改变其细胞周期分布。

Effect of adenosine on proliferation and cell cycle of A549 cells

Objective：To investigate the effect of adenosine on cell proliferation and cell cycle of A549 cells.Methods：A549 cells were cultured,the expression of adenosine receptors in A549 cells were detected by RT-PCR.After A549 cells were treated with 0,1,10,100 and 1 000 μmol/L adenosine for 24,48 and 72 hc,ell viability of each group was measured using MTT.After A549 cells were treated with 100μmol/L for 24 hc,ell cycle was analyzed by flow cytometry.One-way ANOVA or independent samples t-test was used to evaluate the difference of all results among different experimental groups.Results：RT-PCR results showed that four different adenosine receptors（A1,A2A,A2B and A3） expressed in A549 cells.After A549 cells were treated with 0,1,10,100 and 1 000 μmol/L adenosine for 24,48 and 72 h,A549 cells survival rate were 91%,84%,80%,73% at 24 h and 82%,69%,58%,55% at 48 h and 86%,56%,52%,38% at 72 h.With the increase of adenosine concentration,A549 cells proliferation were significant inhibited,which showed the concentra-tion dependent effects.Cell cycle analysis revealed a marked increase in the G1 phase（control group：59.07±0.70;100μmol/L：65.05±0.62;t =-11.04,P 〈0.01）and decrease in the G2 phase（control group：6.67±0.95,100μmol/L：1.29±0.43,t =8.89,P〈 0.01）following 24 h exposure to 100μmol/L adenosine.Conclusion：Adenosine is able to inhibit the proliferation of A549 cells in concen-tration-dependent and time-dependent manners,and can change the cell cycles.