氯霉素对着床前大鼠胚泡的遗传毒性

为评价氯霉素对着床前胚泡的遗传毒性作用，Wistar大鼠在妊娠d3ip氯霉素65mg／kg，165mg／kg，330mg／kg。孕鼠于妊娠d4处死取胚泡，观察微核率，具微核胚泡率及有关丝分裂指数等。结果表明氛霉素各剂量组的微孩率呈剂量依赖性增加，与对照组相比较均有极显著差异；具微核胚烟率与对照组相比无显著差异，但呈一定量效相关性；ip氯霉素330mg/kg时．平均细胞数显著减少，有丝分裂指数与对照相比较无显著差异。提示氯霉素对整体大鼠着床有胚胞具遗传毒性。

Genetic Toxicity of Chloramphenicol to Rat Preimplant Blastocysts in vivo

To evaluate the genetic toxicity of chloramphenicol on preimplanted blastocysts .Wistar rats were treated with chloramphenicol (ip, 65mg, 165mg and 330mg/kg)on d3 of gestationin rats. Blastocysts were collected on d4 and evaluated for micronuclei, blastocysts with micronuclei and embryo mitosis index. The results showed that ,compared with the control, the, whcronucleus frequency has a dose -related increase with significant difference. If treated with chloramphenicol(ip, 330mg/kg),the mlan cell number per blastocyst decreased with significant difference. No markedly difference in the frequency of blastocysts with micronuclci was observed,but there was a increasing trend depending dose. The embryo mitosis index had no significantly difference between the treated and control groups. Results suggest that there may be the effect of genetic toxicity of chloramphenicol on rat preimplanted blastocysts in vivo.