同种异体骨髓间充质干细胞移植对心梗大鼠心脏结构与功能的影响

目的 探讨同种异体骨髓间充质干细胞移植对急性心肌梗死大鼠心脏结构及功能的影响.方法 取SD大鼠30只,随机分成3组,即正常组、模型组和干细胞组,每组10只.正常组不予处理,模型组结扎大鼠左冠状动脉制成急性心肌梗死模型,干细胞组在结扎冠状动脉后向心肌内注射干细胞,4周后检测大鼠心功能及心脏组织病理变化.结果 模型组大鼠心脏左室舒张末容积、左室收缩末容积、舒张末期容积较正常组及干细胞移植组均明显增高,差异具有显著性(P<0.05);模型组大鼠心脏左室射血分数、左室短轴缩短率、心输出量较正常组及干细胞移植组明显降低,差异具有显著性(P<0.05).病理结果显示,模型组见肌纤维溶解甚至消失,可见纤维组织增生瘢痕形成.干细胞组可见心肌纤维排列较正常心肌纤维排列混乱,也有少量纤维组织增生和瘢痕形成.结论 同种异体骨髓间充质干细胞移植能够改善急性心肌梗死模型大鼠心脏的结构与功能.

Effect of Allogeneic Bone Marrow Mesenchymal Stem Cell Transplantation on Cardiac Structure and Function of Myocardial Infarction Rats

Objective To investigate the effect of allogeneic bone marrow mesenchymal stem cell transplantation on the cardiac structure and function in rats with acute myocardial infarction. Methods Thirty SD rats were randomly divided into blank group, model group, and stem cells group, 10 rats in each group. The model group received left coronary artery ligation to induce acute myocardial infarction, and the stem cells group received myocardial injection of stem cells after coronary artery ligation. After four weeks, cardiac function and heart tissue pathological changes were observed. Results In the model group, left ventricular end-diastolic volume, left ventricular end-systolic volume and end dias-tolic volume were increased, and left ventricular ejection fraction, left ventricular fractional shortening rate and cardiac output were decreased as compared with the normal group and the stem cells group (P < 0. 05). The results of pathologi-cal examination showed that myocardiac fibers dissolved or even disappeared, and fibric proliferation and sear occurred in the model group; in the stem cells group, the arrangement of myocardiae fibers was in disorder and there were a few pro-liferated fibers and scar compared with the normal group. Conclusion Allogeneic bone marrow mesenchymal stem cell transplantation can improve the cardiac structure and function in rat model of acute myocardial infarction.