弥漫性脑损伤诱发的前脑胆碱能神经元变化

目的 本实验通过实验性研究，以了解弥漫性脑损伤（ＤＢＩ）是否引起胆碱能神经元的显性减少。方法 采用Ｍａｒｍａｒｏｕ打击装置建立ＤＢＩ动物模型，行胆碱酯酶组织化学染色以显示基底前胆碱能神经元。结果 ①基底前脑胆碱能神经元在重伤组、轻伤组、对照组有显差异（Ｐ〈０．０１）；②损伤后二周组神经元减少与一周组比较有显差异（Ｐ〈０．０１或Ｐ〈０．０５）。结论 ①在本实验所观察到的ＤＢＩ后基底前脑胆碱能神

Changes of Basal Forebrain Cholinergic Neurons after Diffuse Brain Injury in Rats

Objective The purpose of this study was to see whether there was a significant loss of forebrain cholinergic neurons after experimental diffuse brain injury(DBI). Methods Fifty adult Wistar rats were used in this experimental study. The DBI animal models were prepared in 40 rats according to Marmarou method, in which mild DBI was made in 20 rats and severe in the remaining 20. Another 10 rats served as control. Morphological studies of cholinergic neurons were performed in serial sections using acetylcholinesterase (AchE) histochemistiy staining method. Results In comparism with the control group, the number of cholinerginc neurons was significantly decreased in all the rats with DBI (P<0.01) . The decrease in number of cholinergic neurons was more marked in the group where the rats were severely injured and in the group where the rats were sacrificed 2 weeks after injury than that sacrificed at the end of 1 week. Conclusions Decrease in the basal forebrain cholinegic neurons is probably one of the important patholgical changes in cognitive dysfunction after diffuse brain injury.