ApoAI 基因rs12721026多态性与长沙汉族人群脑出血的关系及其对血脂的影响

目的: 探讨载脂蛋白AI(apolipoprotein AI,ApoAI)基因rs12721026单核苷酸多态性与长沙地区汉族人群脑出血及血脂水平的关系。方法: 利用单碱基延伸技术与DNA测序结合相互印证的方法检测长沙地区汉族人群273例脑出血患者及140例正常对照的ApoAI基因rs12721026单核苷酸多态性。采用氧化酶法测定三酰甘油(TG)、总胆固醇(TC)、HDL-C及LDL-C水平。结果: 脑出血组ApoAI基因rs12721026多态基因型及等位基因频率分布与对照组比较差异无统计学意义(P>0.05);脑出血组及对照组TT基因型亚组的HDL-C水平明显高于GT/GG基因型亚组(P<0.05),两组不同基因型亚组间TG,TC,LDL-C水平差异无统计学意义(P>0.05)。结论: ApoAI基因rs12721026多态性可能与长沙地区汉族人群脑出血的发病无关,但可能影响其HDL-C水平。

Association of ApoAI gene rs12721026 polymorphism with cerebral hemorrhage in Changsha Han population and its effect on plasma lipid levels

Objective: To explore the association between apolipoprotein AI (ApoAI) gene rs12721026 polymorphism and cerebral hemorrhage (CH) in Changsha Han population, and to evaluate the effect of rs12721026 polymorphism on plasma lipid levels.
Methods: A total of 273 patients with CH and 140 healthy controls were collected. The rs12721026 polymorphism of ApoAI was analyzed by SNaPshot genotyping analysis and DNA sequencing. The total cholesterol (TG), triglyceride (TC), HDL-C and LDL-C were examined by oxidase method.
Results: There was no significant difference in the genotype and allele frequencies of rs12721026 polymorphism between the CH group and the control group (P>0.05). Both in the CH group and in the control group, the level of HDL-C of the TT gene type of rs12721026 was significantly higher than that of the GT/GG gene type (P<0.05). There was no significant difference in the levels of TG, TC and LDL-C among different subgroups of gene types.
Conclusion: There may be no association between apoAI gene rs12721026 polymorphism with CH in Changsha Han population, which may still influence the HDL-C levels.