人工虎骨粉对去卵巢大鼠骨组织中Sirt1/Runx2信号通路的影响

目的探讨人工虎骨粉对去卵巢大鼠骨组织中Sirt1/Runx2信号通路的影响。方法选取40只成年雌性SD大鼠,建立去卵巢大鼠模型,切除卵巢2周后分成人工虎骨粉组与去卵巢组,人工虎骨粉组给予灌胃人工虎骨粉0. 24 g/(kg·d),去卵巢组给予灌胃等效剂量的生理盐水。另取20只成年雌性SD大鼠作为假手术组,仅切除卵巢周围脂肪组织,不切除双侧卵巢,切除卵巢周围脂肪组织2周后给予灌胃等效剂量的生理盐水。连续给药12周后,进行股骨骨密度、骨代谢、骨生物力学、股骨组织学与Western Blot检测。结果与假手术组比较,去卵巢组股骨骨密度、最大载荷、断裂载荷、最大应力、弹性模量及股骨组织Sirt1、Runx2、Foxo1、Foxo3a蛋白表达降低,骨钙素、抗酒石酸酸性磷酸酶、血钙、血磷及碱性磷酸酶浓度升高,股骨组织呈现骨质疏松病理改变;与去卵巢组比较,人工虎骨粉组股骨密度、最大载荷、断裂载荷、最大应力、弹性模量及股骨组织Sirt1、Runx2、Foxo1、Foxo3a蛋白表达升高,骨钙素、抗酒石酸酸性磷酸酶、血钙、血磷及碱性磷酸酶浓度均降低,骨质疏松病理改变显著改善。结论人工虎骨粉可能通过调节Sirt1/Runx2信号通路来发挥抗骨质疏松作用。

Effects of artificial tiger bone powder on Sirt1/Runx2 signaling pathway in bone tissue of ovariectomized rats

Objective To investigate the effect of artificial tiger bone powder on Sirt1/Runx2 signaling pathway in bone tissue of ovariectomized rats.Methods Forty adult female SD rats were selected to establish ovariectomized rat model. After ovariectomy for 2 weeks, they were divided into artificial tiger bone powder group and ovariectomized group. The artificial tiger bone powder group was given 0.24 g/(kg·d) by gavage, and the ovariectomized group was given equivalent dose of saline by gavage. In addition, 20 adult female SD rats were taken out, only the adipose tissue around ovary was removed, and bilateral ovaries were not removed. After 2 weeks of resection, the adipose tissue around ovary was given the equivalent dose of saline by intragastric administration. After 12 weeks of continuous administration, femoral density, bone metabolism, bone biomechanics, femoral histology and Western Blot were measured.Results Compared with the sham-operated group, the femoral density, maximum load, fracture load, maximum stress, elastic modulus and the expressions of Sirt1, Runx2, Foxo1 and Foxo3a protein in femoral tissue of ovariectomized group decreased, while the concentrations of osteocalcin, tartrate-resistant acid phosphatase, serum calcium, phosphorus and alkaline phosphatase increased, and the femoral tissue presented osteoporosis. Physical changes; Compared with ovariectomized group, femoral density, maximum load, fracture load, maximum stress, elastic modulus and expressions of Sirt1, Runx2, Foxo1 and Foxo3a in femoral tissue of artificial tiger bone powder group increased, while osteocalcin, tartrate-resistant acid phosphatase, serum calcium, phosphorus and alkaline phosphatase decreased, and osteoporosis decreased. Pathological changes were significantly improved.Conclusion Artificial tiger bone powder may play an anti-osteoporosis role by regulating Sirt1/Runx2 signaling pathway.