α-苦瓜素对人正常肝细胞细胞因子表达的调节作用

目的探索α-苦瓜素（α-MMC）对肝细胞细胞因子合成与分泌的调节作用。方法以质量浓度为189 μg/mL的α-MMC加入体外培养的肝细胞L02中，分别于药物作用0 h、2 h、4 h和8 h后收获培养上清和细胞裂解液样品，以Bio-Plex 200悬液芯片检测系统检测各样品中辅助性T细胞17（TH17）细胞因子谱的表达量。结果与0 h相比，α-MMC作用L02肝细胞2 h、4 h和8 h后，细胞内合成的白介素（IL）-1b、IL-6、IL-17A、IL-31、IL-33、可溶性CD40配体（sCD40L）、肿瘤坏死因子-α(TNF-α)等7种细胞因子降低（P＜0.05），分泌至细胞外液的IL-6、IL-4、IL-17A和sCD40L也降低（P＜0.05）。结论α-MMC能抑制肝细胞IL-6、IL-17A和TNF-α等细胞因子的合成与分泌，可能在抗肿瘤的应用中产生副作用。

Alpha-momordicin Regulates Hepatocyte Cytokine Expression

ObjectiveTo investigate the regulation effect of α-momordicin (α-MMC) on the synthesis and secretion of cytokines in hepatocytes cells.MethodsHepatocytes L02 were treated with 189 μg/mL α-MMC with culture supernatant and lysate samples were harvested in different timepoint. Expressions of T-helper 17 (TH17) cytokine profile in samples were detected by the Bio-Plex 200 suspension chip assay system. ResultsCompared with 0 h,after the α-MMC treatment of L02 hepatocytes for 2 h,4 h and 8 h,the intracellular synthesis of cytokines interleukin (IL)-1b,IL-6,IL-17A,IL-31,IL-33,soluble CD40 ligand (sCD40L),tumor necrosis factor-α (TNF-α) were all significantly decreased (P<0.05),and IL-6,IL-4,IL-17A,and sCD40L secreted into the extracellular fluid also decreased significantly (P<0.05). Conclusionα-MMC can significantly inhibit the synthesis and secretion of cytokines such as IL-6,IL-17A and TNF-α in hepatocytes,which may become a side effect of its anti-tumor application.