Elevated level of endothelin-1 in cerebrospinal fluid and lack of nitric oxide in basilar arterial plasma associated with cerebral vasospasm after subarachnoid haemorrhage in rabbits |
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Authors: | Volker Neuschmelting Serge Marbacher Ali-Reza Fathi Stephan M Jakob Javier Fandino |
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Institution: | (1) Department of Neurosurgery, University Hospital Berne, Berne, Switzerland;(2) Department of Intensive Care Medicine, University Hospital Berne, Berne, Switzerland;(3) Department of Neurosurgery, Kantonsspital Aarau, Tellstrasse, 5001 Aarau, Switzerland |
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Abstract: | Background The role of endothelin-1 (ET-1) and nitric oxide (NO) as two important mediators in the development of cerebral vasospasm
(CVS) after subarachnoid haemorrhage (SAH) is controversial. The objective of this study was to determine whether local levels
of ET-1 and NO in cerebral arterial plasma and/or in cerebrospinal fluid (CSF) are associated with the occurrence of CVS after
SAH.
Methods CVS was induced using the one-haemorrhage rabbit model and confirmed by digital subtraction angiography of the rabbits’ basilar
artery on day 5. Prior to sacrifice, local CSF and basilar arterial plasma samples were obtained by a transclival approach
to the basilar artery. Systemic arterial plasma samples were obtained. ET-1 levels were determined by immunometric technique
(pg/ml ± SEM) and total nitrate/nitrite level spectrophotometrically (μmol/l ± SEM).
Findings Angiographic CVS was documented after SAH induction (n = 12, P < 0.05). The ET-1 level in CSF was significantly elevated by
27.3% to 0.84 ± 0.08 pg/ml in SAH animals (n = 7) in comparison to controls (0.66 ± 0.04 pg/ml, n = 7, P < 0.05). There was
no significant difference in ET-1 levels in systemic and basilar arterial plasma samples of SAH animals compared to controls.
A significant lack of local NO metabolites was documented in basilar arterial plasma after SAH (36.8 ± 3.1 μmol/l, n = 6)
compared to controls (61.8 ± 6.2 μmol/l, n = 6, P < 0.01).
Conclusion This study demonstrates that an elevated ET-1 level in CSF and local lack of NO in the basilar arterial plasma samples are
associated with CVS after experimental SAH. |
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Keywords: | Subarachnoid haemorrhage Cerebral vasospasm Endothelin-1 Nitric oxide |
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