NLRP3炎性小体在嗜酸粒细胞性鼻息肉发病及复发中的作用

目的 探讨NLRP3炎性小体在嗜酸粒细胞性鼻息肉(ENP)发病及复发中的可能作用及机制。 方法 根据HE染色,选取80例ENP(ENP组)、24例嗜酸粒细胞性复发鼻息肉(RENP)患者(RENP组)的鼻息肉组织及同期30例鼻中隔偏曲患者(对照组)的中鼻甲黏膜。所有患者嗜酸粒细胞(EOS)浸润数通过HE染色观察并计算,采用免疫组化法检测3组NLR含热蛋白结构域3(NLRP3)炎性小体、白细胞介素-1beta(IL-1β)、IL-18的表达情况,并分析ENP、RENP组中NLRP3炎性小体与EOS浸润数的相关性。 结果 IL-1β和IL-18在ENP及RENP组中的表达均高于对照组(P<0.05),但在ENP与RENP组中的表达无显著差异(P>0.05)。NLRP3炎性小体在RENP组中的表达明显高于ENP组和对照组(P<0.01),且EOS浸润数明显多于ENP组(P<0.05)和对照组(P<0.01)。NLRP3炎性小体在ENP及RENP组中的表达均与EOS浸润数呈正相关。 结论 NLRP3炎性小体、IL-1β和IL-18均与ENP发病密切相关,NLRP3炎性小体可能参与ENP的复发。

Effect of NLRP3 inflammasome in the pathogenesis and relapse of eosinophilic nasal polyps

Objective To explore the possible effect and mechanism of NLRP3 inflammasome in the pathogenesis and re-lapse of eosinophilic nasal polyps (ENP).Methods According to HE staining,the nasal polyp tissues from 80 cases of ENP (ENP group)and 24 cases of recurrent essinophilic nasal polyps (RENP,RENP group)as well as middle turbinate mucosa from 30 cases of deviated nasal septum (control group)were selected.The number of eosinophil (EOS)infiltra-tion in all patients was detected and observed by HE staining.The immunohistochemistry was used to detect the expression of NLRP3 inflammasome,IL-1βand IL-18 in three groups,and the correlation between NLRP3 inflammasome and the number of EOS infiltration was analyzed in ENP and RENP groups.Results The expression of IL-1βand IL-18 in both ENP and RENP groups was higher than in control group (P <0.05),but no significant difference was presented between ENP group and RENP group (P >0.05).The expression of NLRP3 inflammasome in RENP group was higher significant-ly than in ENP group and control group (P <0.01),and the number of EOS infiltration more than ENP group (P <0.05)and control group (P <0.01).The expression of NLRP3 inflammasome in both ENP and RENP groups positively correlated with the number of EOS infiltration.Conclusion NLRP3 inflammasome,IL-1βand IL-18 were all associated with the pathogenesis of ENP closely,and NLRP3 inflammasome may involve in the relapse of ENP.