| 缝隙连接蛋白43对双自杀基因系统旁观者效应的影响 |
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| 引用本文： | 董泾青,左石,刘茂玲,甘燕,陈波,邹声泉.缝隙连接蛋白43对双自杀基因系统旁观者效应的影响[J].中德临床肿瘤学杂志,2006,5(2):108-112. |
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| 作者姓名： | 董泾青 左石 刘茂玲 甘燕 陈波 邹声泉 |
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| 作者单位： | [1]Departmeat of General Surgery, Affiliated Tongji Hospital, Tongji Medical College~ Huazhong University of Science andTechnology, Wuhan 430030, China [2]Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| 摘 要： | Objective： To observe the influence of connexin 43 （Cx43） on the bystander effect induced by cytosine deaminase （CD） and herpes simplex virus thymidine kinase （HSV-tk） coexpression suicide genes system in human cholangiocarcinoma QBC939 cells and transplantation tumors in nude mice. Methods： In vitro, the CD＋tk＋ and CD＋tk＋Cx＋ cells were respectively treated with 5-fluorocytosine （5-Fc） and Ganciclovir （GCV）. The cytotoxic effect was evaluated by MTT method. In order to investigate the influence of Cx43 on the bystander effect, the size of transplantation tumors of the CD＋tk＋ and CD＋tk＋Cx＋ cells was measured before and after application of 5-Fc and GCV. Results： CD and tk genes were stably expressed in transfected QBC939 cells. The increased expression of Cx43 was determined by testing for the presence of Cx43 mRNA by RT-PCR and the presence of Cx43 protein by Western Blot in CD＋tk＋Cx＋ cells. The killing effect of 5-Fc and GCV on CD＋tk＋Cx＋ cells was more effective than that on CD＋tk＋ cells both in vitro and in vivo. Conclusion： Double suicide genes system CD/5-Fc＋tk/GCV could induce remarkable killing effect on cholangiocarcinoma cells in vitro and transplantation tumors in vivo. The cotransfection of Cx43 gene could enhance the bystander effect and hence the inhibition of carcinoma cells.
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| 关 键 词： | 缝隙连接蛋白43 自杀基因 旁观者效应 胆管肿瘤 基因疗法 |
| 收稿时间： | 2005-02-05 |
| 修稿时间： | 2005-02-28 |
Influence of Connexin43 on the Bystander Effect Induced by
Double Suicide Genes System in Vitro and in Vivo |
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| Jingqing DONG,Shi ZUO,Maoling LIU,Yan GAN,Bo CHEN,Shengquan ZOU.Influence of Connexin43 on the Bystander Effect Induced by
Double Suicide Genes System in Vitro and in Vivo[J].The Chinese-German Journal of Clinical Oncology,2006,5(2):108-112. |
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| Authors: | Jingqing DONG Shi ZUO Maoling LIU Yan GAN Bo CHEN Shengquan ZOU |
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| Institution: | (1) Departmeat of General Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China;(2) Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China |
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| Abstract: | Objective: To observe the influence of connexin 43 (Cx43) on the bystander effect induced by cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-tk) coexpression suicide genes system in human cholangiocarcinoma QBC939 cells and transplantation tumors in nude mice. Methods: In vitro, the CD+tk+ and CD+tk+Cx+ cells were respectively treated with 5-fluorocytosine (5-Fc) and Ganciclovir (GCV). The cytotoxic effect was evaluated by MTT method. In order to investigate the influence of Cx43 on the bystander effect, the size of transplantation tumors of the CD+tk+ and CD+tk+Cx+ cells was measured before and after application of 5-Fc and GCV. Results: CD and tk genes were stably expressed in transfected QBC939 cells. The increased expression of Cx43 was determined by testing for the presence of Cx43 mRNA by RT-PCR and the presence of Cx43 protein by Western Blot in CD+tk+Cx+ cells. The killing effect of 5-Fc and GCV on CD+tk+Cx+ cells was more effective than that on CD+tk+ cells both in vitro and in vivo. Conclusion: Double suicide genes system CD/5-Fc+tk/GCV could induce remarkable killing effect on cholangiocarcinoma cells in vitro and transplantation tumors in vivo. The cotransfection of Cx43 gene could enhance the bystander effect and hence the inhibition of carcinoma cells. |
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| Keywords: | connexin 43 suicide gene bystander effect bile duct tumor gene therapy |
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