丹参对兔缺血再灌注肾组织一氧化氮及其合成酶的影响

目的：探讨丹参保护兔缺血再灌注肾损伤的内皮机制。方法：以兔肾缺血再灌注为模型，采用自动生化分析技术，检测不同实验条件下肾组织一氧化氮合成酶（NOS）总活性及一氧化氮（NO），并用Elasa法测定尿视黄醇结合蛋白（RBP）及尿微量白蛋白（Alb) ,试图分析NOS总活性，NO与肾功能之间的相互关系。结果：（1）正常肾髓质较皮质能产生更多的NO。（2）缺血肾组织NOS总活性显著下降，NO显著增高。（3）再灌注后，肾组织NOS总活性显著升高，而NO显著下降，肾功能明显受损。（4）肾缺血注射丹参后再灌注，肾组织NOS总活性进一步升高，而NO变化不明显，尿RBP及Abl下降至正常水平。结论：（1）生理情况下，肾髓质较皮质能产生更多的NO，这对肾髓质血流分布及其功能的调节可能具有重要作用。（2）缺血再灌注后，肾组织NOS活性升高与肾功能受损密切相关。（3）丹参对缺血再灌注所致的肾小管及肾小球损伤均有明显的保护作用。（4）丹参对缺血再灌注肾组织中的NO具有抑制作用。

Influence of Salvia Miltiorrhiza(SM) on nitric oxide and its synthase of rabbit kidney exposed to ischemia reperfusion injury

Objective To investigate the Influence of Salvia Miltiorrhiza(SM) on nitric oxide(NO) of rabbit kidney exposed to ischemia reperfusion(I/R) injury. Methods A model of rabbit renal I/R was established. Nitric oxied synthase(NOS) activity, NO of renal tissues, Ueronal Alb and RBP were detected. Results In normal kidney, NO of renal medulla was much more than that of renal cortex. When renal ischemia occurred, NOS activity of renal tissues was decreased significantly and accompanied by increasing of NO. After renal reperfusion, NOS activity was increased and NO was decreased significantly, and renal function was damaged obviously. After SM was used, NOS activity of renal tissues had further increasing and ueronal RBP?Alb went down to the normal lever, but NO had no obvious change. Conclusions After renal ischemia reperfusion, the increasing of NOS activity of renal tissues is closely related to the obvious damage of renal function. SM has obviously protective effect on glomerulus and ruinoparous tubules functions after I/R injury. SM can inhibit NO of renal tissues which exposed to ischemia reperfusion injury.